B. Light-repressible promoters
Suntory Ltd.
(http://www.suntory.com/group/suntory_ltd.html)
has patents granted in Australia and New Zealand and pending applications in
Europe and Canada. This patent family mainly claims for a promoter of the
pra2 gene from pea. Mycogen Plant Sciences
(http://www.dowagro.com/mycogen/contact/index.htm)has
two granted United States patents regarding methods for the expression and
enhancing the level of expression of a structural gene in plants by using a
dark- and light-active maize Cab promoter/regulatory system.
Specific Patent Information
|
Patent Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
|
AU
765413 B2
- Earliest priority - 12 March 1999
- Filed - 3 March 2000
- granted - 18 September 2003
- Expected expiry - 3 March 2020
|
Title - Photoinhibitory promoter
Claim 1
A DNA fragment containing the sequence of SEQ ID NO: 1 as a
core sequence, whereby expression of a gene placed downstream of said DNA
fragment is repressed in the presence of light.
|
Claim 4
A promoter containing the sequence of SEQ ID NO: 1 as a core
sequence, whereby expression of a gene placed downstream of said promoter is
promoted in the dark but repressed in the presence of light.
|
The claims are drawn to a promoter sequence or a fragment of the promoter
sequence whose ability of controlling the expression of a gene placed downstream
is promoted in the dark but repressed by light.
The promoter was isolated from a small G protein gene
pra2 from pea. The pra2 gene was thought to be
involved in the elongation of stems, at the epicotyl, during germination in the
dark.
|
Suntory Ltd.
|
|
NZ 508103 B
- Earliest priority - 12 March 1999
- Filed - 3 March 2000
- granted - 28 August 2002
- Expected expiry - 3 March 2020
|
Title - Light-repressible promoters
The claims are exactly the same as
AU
765413.
|
|
Remarks
|
Related patent applications are pending in Europe (EP 1077257
A1) and Canada (CA 2328139 A1).
|
|
US
5639952
- Earliest priority - 5 January 1989
- Filed - 7 April 1995
- Granted - 17 June 1997
- Expected expiry - 17 June 2014
|
Title - Dark and light regulated chlorophyll A/B binding
protein promoter-regulatory system
Claim 1
A method for the expression of a structural gene in a plant cell under
conditions of darkness comprising the steps of:
a. transforming said plant cell with a recombinant molecule
comprising a plant, dark- and light-active maize Cab
promoter/regulatory system, wherein said promoter/regulatory system is the
promoter/regulatory system of Cab AB1084 or cross-hybridizes with the
promoter/regulatory system of Cab AB1084 under stringent conditions, and a
heterologous plant-expressible structural gene that is under the regulatory
control of said plant Cab promoter/regulatory system in said plant cell, and
b. maintaining conditions of darkness for expression of said structural gene
in said transformed plant cell.
|
Claim 8
A method for enhancing the level of expression obtained in the dark of a
plant-expressible gene in a plant cell by exposing said plant cell to conditions
of illumination comprising the steps of:
a. transforming said plant cell with a recombinant molecule
comprising a plant, dark- and light-active maize Cab
promoter/regulatory system, wherein said promoter/regulatory system is the
promoter/regulatory system of Cab AB1084 or cross-hybridizes with the
promoter/regulatory system of Cab AB1084 under stringent conditions, and a
heterologous plant-expressible structural gene that is under the regulatory
control of said plant Cab promoter/regulatory system in said plant cell, and
b. maintaining conditions of darkness for expression of said structural gene
in said transformed plant cell, and
c. applying conditions of illumination to said transformed plant cell such
that enhancement by a factor of about 3- to 6-fold in expression of said
structural gene is obtained.
|
Claim 15
A plant grown from a plant cell, wherein said plant cell is transformed with
a recombinant molecule comprising a plant, dark- and light-active maize Cab
promoter/regulatory system, wherein said promoter/regulatory system is the
promoter/regulatory system of Cab AB1084 or cross-hybridizes with the
promoter/regulatory system of Cab AB1084 under stringent conditions, and a
heterologous plant-expressible structural gene that is under the regulatory
control of said plant Cab promoter/regulatory system in said plant cell.
|
This patent mainly claims methods for the expression of a structural gene in
a plant under dark conditions by placing the gene under the control of a dark
and light-active maize chlorophyll a/b (Cab) promoter/regulatory system. The
promoter/regulatory system used is either the Cab AB1084 or cross-hybridizes
under stringent conditions. The expression of the gene under control of the
system is further enhanced by a subsequent period of light. A plant grown from a
plant cell transformed with such promoter/regulatory system is also part of the
claimed invention.
|
Mycogen Plant Sciences
|
|
US
5656496
- Earliest priority - 5 January 1989
- Filed - 5 June 1995
- granted 12 August 1997
- Expected expiry - 12 August 2014
|
Title - Dark and light regulated chlorophyll A/B binding
protein promoter-regulatory system
Claim 1
A recombinant DNA molecule comprising:
(a) a dark- and light-active Cab promoter/regulatory system which
cross-hybridizes with the Cab AB1084 promoter/regulatory system under stringent
conditions, and wherein said promoter/regulatory system
(i) functions to direct the expression of a structural gene under its
control during conditions of darkness and
(ii) is stimulated to direct enhanced expression of said structural gene
under light conditions, and
(b) a heterologous plant-expressible structural gene wherein said structural
gene is placed under the regulatory control of said plant promoter/regulatory
system.
|
This US patent is a division of
US
5639952. It is directed to a dark and light-active
Cab promoter/regulatory system, which cross-hybridizes with the
Cab AB1084. The promoter system operates the same as described
in
US
5639952.
|
Note: Patent information was last updated on 12 May 2006.
Search terms for Suntory patents: "promoter" in title or abstract and "Suntory"
in applicant. Patent database: esp@cenet worldwide.
Search terms for Mycogen patents: "promoter" in abstract and "Mycogen" in
applicant. Patent database: PatentLens in combination with INPADOC.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
Corrections or updates to the information are welcome. Please send an email to
info@bios.net.
There are no comments.