Temperature-regulated promoters
IP issues
1. Heat-inducible promoters
Mycogen Plant Sciences, The United States Department of Health and Human
Services and The General Hospital Corporation have granted patents and patent
applications that relate in general to DNA sequences of heat shock promoters and
methods for expressing a gene of interest under the control of such promoters.
Some of the inventions relate to the use of the heat shock promoters in
transformed plants, while others do not specify the organism to be transformed.
For some properties of heat shock promoters please also refer to
the plant
ubiquitin promoters.
A. Mycogen Plant Sciences' patents
The patents granted to Mycogen in the United States, Europe and Canada are
directed to:
- consensus DNA sequences of plant heat shock promoters;
- bacterial strains containing a vector with a consensus heat shock promoter
and a gene of interest under its control for expression in plants;
- recombinant Agrobacterium Ti-plasmids comprising a heat shock
promoter;
- methods for expressing a heterologous gene under the control of a heat shock
promoter in plant cells; and
- methods for identifying transformed plants.
patent claims in particular the use of a soybean heat shock promoter
to drive the expression of a structural gene in a plant. The soybean
heat shock promoter and the structural gene are engineered in a plasmid carried
by an Agrobacterium strain used to infect the plant to be transformed.
Mycogen Plant Sciences has changed its name to Mycogen Seeds and is now an
affiliate of Dow AgroSciences LLC. Therefore , Mycogen patents and patent
applications are probably controlled by Dow AgroSciences LLC. The contact
information can be found at http://www.dowagro.com/contact/index.htm or
http://www.dowagro.com/mycogen/contact/index.htm.
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5447858
- Earliest priority - 5 September 1984
- Filed - 5 September 1984
- Granted - 5 September 1995
- Expected expiry - 5 September 2012
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Title - Heat shock promoter and gene
Claim 1
A recombinant heat shock gene comprising a plant heat shock
promoter and a heterologous structural gene whose expression is controllable
thereby, wherein said promoter comprises the consensus
nucleotide sequence 5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A,T,C or G.
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Claim 2
A recombinant heat shock gene comprising a plant heat shock
promoter and a heterologous structural gene whose expression is controlled
thereby, wherein said promoter comprises the consensus
nucleotide sequence 5'-C-T-S-G-A-A-M-R-T-A-C-W-M-K-3' where S is C or G; M is A
or C; R is A or G; W is A or T; and K is T or G.
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Claim 8
A vector comprising a fragment of DNA capable of functioning
as a plant heatshock promoter wherein said fragment of DNA
comprises the consensus nucleotide sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C cr G.
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Claim 9
A vector comprising a fragment of DNA capable of functioning
as a plant heat shock promoter wherein said fragment comprises
the consensus nucleotide sequence of 5'-C-T-S-G-A-A-M-R-T-A-C-W-M-K-3' where S
is C or G; M is A or C; R is A or G; W is A or T; and K is T or G.
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Claim 19
A bacterial strain containing therein recombinant DNA
comprising:
(a) a fragment of DNA capable of
controlling heatshock expression of a gene in a plant; and
(b) a
structural gene oriented with respect to said fragment of DNA so as to be
expressible under the control thereof wherein said fragment of DNA capable of
controlling heat shock expression of a gene in a plant
comprises the consensus nucleotide sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C or G.
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Claim 20
A bacterial strain containing therein recombinant DNA
comprising:
(a) a fragment of DNA capable of
controlling heat shock expression of a gene in a plant; and
(b) a
structural gene oriented with respect to said fragment of DNA so as to be
expressible under the control thereof wherein the fragment of DNA capable of
controlling heat shock expression of a gene in a plant
comprises the consensus nucleotide sequence
5'-C-T-S-G-A-A-M-R-T-A-C-W-M-K-3' where S is C or G; M is A or C; R is A or G; W
is A or T; and K is T or G.
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Claim 34
A method for recognizing a plant cell containing recombinant DNA
comprising the steps of:
(1) transferring into said
plant cell recombinant DNA comprising:
(a) a heat shock promoter
which comprises the consensus sequence 5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where
X is A, T, C, or G;
(b) a transformation recognition gene under
the control of said heat shock promoter capable of causing an observable or
detectable reaction when expressed;
(2) applying a stress to said
plant cell in which it is desired to recognize those containing recombinant DNA,
which stress is capable of inducing a response in said heat shock promoter; and
(3) observing or detecting the reaction caused by expression of said
transformation recognition gene under the control of said heat shock promoter to
recognize said plant cell containing recombinant DNA including said
transformation recognition gene.
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Claim 36
A method for recognizing a plant cell containing recombinant DNA
comprising the steps of:
(1) transferring into said
plant cell recombinant DNA comprising:
(a) a heat
shock promoter which comprises the consensus sequence
5'-C-T-S-G-A-A-M-R-T-A-C-W-M-K-3' where S is C or G; M is A or C; R is A or G; W
is A or T; and K is T or G;
(b) a transformation recognition gene
under the control of said heat shock promoter capable of causing an observable
or detectable reaction when expressed;
(2) applying a stress to said
plant cell in which it is desired to recognize those containing recombinant DNA,
which stress is capable of inducing a response in said heat shock promoter; and
(3) observing or detecting the reaction caused by expression of said
transformation recognition gene under the control of said heat shock promoter to
recognize said plant cell containing recombinant DNA including said
transformation recognition gene.
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Mycogen Plant Sciences
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EP
159884 B1
- Earliest priority - 13 April 1984
- Filed - 12 April 1985
- Granted - 10 February 1993
- Expected expiry - 12 April 2005
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Title - Heat shock promoter and gene
The claims are the same as
US
5447858 (see above).
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CA 1338010
- Earliest priority - 13 April 1984
- Filed - 11 April 1985
- Granted - 30 January 1996
- Expected expiry - 29 January 2013
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Title - Heat shock promoter and gene
This Canadian patent claims in particular the use of a soybean heat
shock promoter to drive the expression of a structural gene in a plant.
The soybean heat shock promoter and the structural gene are engineered in a
plasmid carried by an Agrobacterium strain used to infect the plant to
be transformed.
Claim 1
A recombinant heat shock gene comprising a plant heat shock
promoter and a heterologous structural gene whose expression is controllable
thereby, wherein said promoter comprises the consensus
nucleotide sequence 5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A,T,C or G.
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Claim 8
A vector comprising a fragment of DNA capable of functioning
as a plant heatshock promoter wherein said fragment of DNA
comprises the consensus nucleotide sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C cr G.
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Claim 19
A bacterial strain containing therein recombinant DNA
comprising:
(a) a fragment of DNA capable of
controlling heatshock expression of a gene in a plant; and
(b) a
structural gene oriented with respect to said fragment of DNA so as to be
expressible under the control thereof wherein said fragment of DNA capable of
controlling heat shock expression of a gene in a plant
comprises the consensus nucleotide sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C or G.
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Claim 34
A method for obtaining a transiet increase in expression level of a
heterologous structural gene in a plant comprising
inserting into said genome a DNA fragment comprising a promoter
capable of inducing a heat shock reaponse in a plant exposed to stress, which
promoter comprises the consensus nucleotide sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C, or G, said promoter being
combined with said structural gene such that said structural gene is expressed
under the control of said promoter, followed by
exposing said plant to
stress such that said promoter responds thereby obtaining a transient increase
in expression level of said structural gene in said plant.
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Claim 54
A method for recognizing a plant cell containing recombinant DNA
comprising the steps of:
(1) transferring into said
plant cell recombinant DNA comprising:
(a) a heat
shock promoter which comprises the consensus sequence
5'-C-T-X-G-A-A-X-X-T-A-C-X-X-X-3', where X is A, T, C, or G;
(b) a
transformation recognition gene under the control of said heat shock promoter
capable of causing an observable or detectable reaction when expressed;
(2) applying a stress to said plant cell in which it is desired to recognize
those containing recombinant DNA, which stress is capable of inducing a response
in said heat shock promoter; and
(3) observing or detecting the
reaction caused by expression of said transformation recognition gene under the
control of said heat shock promoter to recognize said plant cell containing
recombinant DNA including said transformation recognition gene.
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Remarks
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There are two related applications pending in Japan (JP 60248176
A2 and JP 7075567 A2).
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Note: Patent information on this page was last updated on 4 May 2006.
Search terms: "Heat shock promoter" in abstract; "Mycogen Plant Sciences" in
applicant
Patent database: Patent Lens
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