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Patent/application number
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Title, Independent claims and Summary of the claims
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Assignee
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US
5654414
- Earliest priority - 19 May 1995
- Filed - 19 May 1995
- Granted - 5 August 1997
- Expected expiry - 19 May 2015
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Title - Chemically inducible promoter of a cucumber
chitinase/lysozyme gene
Claim 1
A nucleic acid promoter fragment isolated from the 5' flanking region
upstream of the coding region of a cucumber chitinase/lysozyme gene that is
inducible by application of benzo-1,2,3-thiadiazoles.
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This patent mainly claims for an isolated promoter region of a
cucumber chitinase/lysozyme gene that is inducible by
application of benzo 1,2,3,-thiadiazoles (BTH). Chitinases are
pathogenesis-related enzymes induced in the SAR response.
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Novartis Finance Corporation
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US
5689044
- Earliest priority - 19 May 1995
- Filed - 24 May 1995
- Granted - 18 November 1997
- Expected expiry - 19 May 2015
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Title - Chemically inducible promoter of a plant PR-1 gene
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Claim 1
A chemically inducible nucleic acid promoter fragment isolated from the 5'
flanking region upstream of the coding region of a tobacco
PR-1a gene, wherein said promoter fragment comprises a
nucleotide fragment of at least 603-bp adjacent to the coding region of said
tobacco PR-1a gene, wherein said promoter fragment is inducible by application
of a benzo-1,2,3-thiadiazole, an isonicotinic acid compound, or a salicylic acid
compound.
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Claim 3
A chemically inducible nucleic acid promoter fragment isolated from the 5'
flanking region upstream of the coding region of an
Arabidopsis PR-1 gene, wherein the coding region of
said Arabidopsis PR-1 gene comprises
the DNA sequence set forth in
SEQ
ID NO:33 or a DNA sequence which would encode the protein encoded by
SEQ
ID NO:33, wherein said promoter fragment is inducible by application of a
benzo-1,2,3-thiadiazole, an isonicotinic acid compound, or a salicylic acid
compound.
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The claiming elements are:
- An isolated region of at least 603 bp that constitutes the tobacco
PR-1a gene promoter. The promoter is inducible by application of
salicylic acid, BTH and 2,6-dichloroisonicotinic acid (INA).
- The isolated promoter region of an
Arabidopsis PR-1 gene. This promoter is inducible by
the same compounds as the tobacco PR-1a gene promoter.
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US
5789214
- Earliest priority - 19 May 1995
- Filed - 31 May 1995
- Granted - 4 August 1998
- Expected expiry - 4 August 2015
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Title - Method of inducing gene transcription in a plant
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Claim 1
A method of inducing gene transcription in a plant or plant tissue,
comprising the steps of:
(a) transforming said plant or plant tissue, each with a chimeric gene
comprising:
(i) a chemically inducible nucleic acid promoter fragment of at least
603-bp isolated from the 5' flanking region adjacent the coding region of a
tobacco PR-1a gene, and
(ii) a coding sequence of interest operatively linked to said promoter
fragment; and
(b) exposing said transgenic plant or plant tissue to a
benzo-1,2,3-thiadiazole, an isonicotinic acid compound, or a salicyclic acid
compound, whereby transcription of said coding sequence of interest is induced
in said plant or plant tissue.
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Claim 8
A method of inducing gene transcription in a plant or plant tissue,
comprising the steps of:
(a) transforming said plant or plant tissue, each with a chimeric gene
comprising:
(i) a chemically inducible nucleic acid promoter fragment isolated from
the 5' flanking region adjacent the coding region of an Arabidopsis PR-1 gene,
wherein said Arabidopsis PR-1 gene comprises a DNA sequence that specifically
hybridizes
to SEQ
ID NO:33 or wherein said Arabidopsis PR-1 gene comprises a DNA sequence that
encodes the protein encoded by
SEQ
ID NO:33, and
(ii) a coding sequence of interest operatively linked to said promoter
fragment; and
(b) exposing said transgenic plant or plant tissue to a
benzo-1,2,3-thiadiazole, an isonicotinic acid compound, or a salicylic acid
compound, whereby transcription of said coding sequence of interest is induced
in said plant or plant tissue.
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The claims are mainly to:
- Methods for inducing gene transcription in a plant or a plant tissue by
transforming such plant with a chimeric gene comprising the tobacco
PR-1a gene promoter or the Arabidopsis PR-1
gene promoter linked to a gene of interest and
applying an inducer to the transformed plant. The inducers are selected from
salicylic acid, BTH and isonicotinic acid (INA).
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AU
708850 B2
- Earliest priority - 23 July 1995
- Filed - 18 July 1997
- Granted - 12 August 1999
- Expected expiry - 18 July 2017
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Title - Chemically-inducible Arabidopsis PR-1 promoter
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Claim 1
An isolated DNA molecule comprising a nucleotide sequence
selected from the following group:
a) a full-length chemically inducible promoter fragment
comprising nucleotides 1 through 4258 of SEQ ID NO:1;
b) an 81 5-bp long chemically inducible promoter fragment
comprising nucleotides 3444 through 4258 of SEQ ID NO:1;
and
c) a 698-bp long chemically inducible promoter fragment
comprising nucleotides 3561 through 4258 of SEQ ID NO:1.
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Claim 14
An isolated DNA molecule involved in inducibility of a chemically inducible
promoter selected from the following group:
a) LS4 comprising nucleotides 3584 through 3593 of SEQ ID NO:1;
b) L57 comprising nucleotides 3614 through 3623 of SEQ ID NO:1;
c) LS 10 comprising nucleotides 3644 through 3653 of SEQ ID NO:1; and
d) a region spanning LS7-LSIO and comprising nucleotides 3614 through 3653
of SEQ ID NO:1.
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The claiming elements are:
- Isolated full-length chemically-inducible Arabidopsis PR-1
promoter and isolated shorter portions of the promoter that are
required for induction of gene expression by chemicals such as salicylic acid,
BTH and 2,6-dichloroisonicotinic acid (INA).
- Isolated motifs in the promoter that when mutated alters the inducible
activity of the promoter
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Remarks
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Related applications also filed in Canada (CA 2232741 AA)
and Japan (JP 11513897 T2). The claims as filed of the Canadian
application are the same as the claims of the Australian granted patent. The
related European application EP 868 426 A1 was withdrawn on
January 2, 2003.
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US
6429362
- Earliest priority - 26 February 1998
- Filed - 25 February 1999
- Granted - 6 August 2002
- Expected expiry - 25 February 2019
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Title - Maize PR-1 gene promoters
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Claim 1
An isolated promoter comprising a nucleotide sequence that initiates
transcription in a plant cell, wherein said nucleotide sequence is selected from
the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in
SEQ
ID NO: 3 or
4;
and
b) a nucleotide sequence comprising the plant promoter sequence deposited in
the plasmid designated as ATCC Accession No. 207139 or 207131.
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Claim 5
A method for driving expression of a heterologous nucleotide sequence in a
plant, said method comprising the steps of:
a) transforming a plant cell with an expression cassette comprising the
heterologous nucleotide sequence operably linked to a promoter that initiates
transcription in a plant cell, wherein said promoter is selected from the group
consisting of:
i) a nucleotide sequence comprising the sequence set forth in
SEQ
ID NO: 3 or
4;
and
ii) a nucleotide sequence comprising the plant promoter sequence
deposited in the plasmid designated as ATCC Accession No. 207139 or 207131; and
b) regenerating a stably transformed plant from said plant cell.
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Claim 9
A plant cell transformed with a DNA construct comprising a heterologous
nucleotide sequence operably linked to a promoter that initiates transcription
in said plant cell, wherein said promoter comprises a nucleotide sequence
selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in
SEQ
ID NO: 3 or
4;
and
b) a nucleotide sequence comprising the plant promoter sequence deposited in
the plasmid designated as ATCC Accession No. 207139 or 207131.
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Claim 13
A plant stably transformed with a DNA construct comprising a heterologous
nucleotide sequence operably linked to a promoter that initiates transcription
in a plant cell, wherein said promoter comprises a nucleotide sequence selected
from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in
SEQ
ID NO: 3 or
4;
and
b) a nucleotide sequence comprising the plant promoter sequence deposited in
the plasmid designated as ATCC Accession No. 207139 or 207131.
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The claims are generally drawn to:
- Two isolated DNA sequences of inducible maize PR-1 gene
promoters
- A method to drive the expression of a heterologous gene in a plant by using
the claimed maize PR-1 promoters
- A plant cell transformed with a DNA construct comprising either of the two
promoters, and
- A plant stably transformed with such a construct
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Pioneer Hi-Bred International Inc.
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AU
754376 B2
- Earliest priority - 26 February 1998
- Filed - 11 February 1999
- Granted - 14 November 2002
- Expected expiry - 11 February 2019
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Title - Family of maize PR-1 genes and promoters
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Claim 1
An isolated nucleic acid molecule having a nucleotide sequence for a promoter
that is capable of initiating transcription in a plant cell, wherein said
nucleotide sequence is selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
1,2,3,4, or 5;
b) a nucleotide sequence comprising at least 40 contiguous nucleotides of
the sequence set forth in SEQ ID NO: 1,2,3,4, or 5; and
c) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a) or b).
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Claim 5
A method for inducing expression of a heterologous nucleotide sequence in a
plant, said method comprising transforming a plant cell with a DNA construct
comprising said heterologous nucleotide sequence operably linked to a promoter
that is capable of initiating transcription in a plant cell in response to a
stimulus, regenerating a stably transformed plant from said plant cell, and
exposing said plant to said stimulus, wherein said promoter comprises a
nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID
NO:1,2,3, or 4;
b) a nucleotide sequence comprising at least 40 contiguous nucleotides of
the sequence set forth in SEQ ID NO: 1,2,3, or 4; and
c) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a) or b).
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Claim 9
A method for constitutively expressing a heterologous nucleotide sequence in a
plant, said method comprising transforming a plant cell with a DNA construct
comprising said heterologous nucleotide sequence operably linked to a promoter
that is capable of initiating constitutive transcription in a plant cell and
regenerating a stably transformed plant from said plant cell, wherein said
promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 5;
b) a nucleotide sequence comprising at least 40 contiguous nucleotides of
the sequence set forth in SEQ ID NO:5; and
c) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a) or b).
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Claim 13
A plant cell stably transformed with a DNA construct comprising a heterologous
nucleotide sequence operably linked to a promoter that is capable of initiating
transcription in said plant cell, wherein said promoter comprises a nucleotide
sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
1,2,3,4, or 5;
b) a nucleotide sequence comprising at least 40 contiguous nucleotides of
the sequence set forth in SEQ ID NO: 1,2,3,4, or 5; and
c) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a) or b).
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Claim 17
A plant stably transformed with a DNA construct comprising a heterologous
nucleotide sequence operably linked to a promoter that is capable of initiating
transcription in a plant cell, wherein said promoter comprises a nucleotide
sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
1,2,3,4, or 5;
b) a nucleotide sequence comprising at least 40 contiguous nucleotides of
the sequence set forth in SEQ ID NO: 1,2,3,4, or 5; and
c) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a) or b).
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Claim 22
An isolated nucleic acid molecule having a nucleotide sequence selected from the
group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
6,8,10, or 14;
b) a nucleotide sequence encoding a polypeptide comprising the amino acid
sequence set forth in SEQ ID NO: 7,9,11, or 15;
c) a nucleotide sequence that shares at least 85% sequence identity to the
sequence set forth in SEQ ID NO:6, 8 or 14; and
d) a nucleotide sequence that shares at least 90% sequence identity to the
sequence set forth in SEQ ID NO:10.
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Claim 26
A method for creating or enhancing disease resistance in a plant, said method
comprising transforming said plant with a DNA construct comprising a PR-1
sequence operably linked to a promoter that drives expression of a coding
sequence in a plant cell and regenerating stably transformed plants, wherein
said PR-1 sequence is selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set fortha in SEQ ID NO: 6,
8, 10, or 14;
b) a nucleotide sequence encoding a polypeptide comprising the amino acid
sequence set forth in SEQ ID NO: 7, 9, 11, or 15;
c) a nucleotide sequence comprising at lease 16 contiguous nucleotides of a
sequence of a) or b); and
d) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a), b), or c).
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Claim 33
A plant cell stably transformed with a DNA construct comprising a PR-1 sequence
operably linked to a promoter that drives expression of a coding sequence plant
cell, wherein said PR-1 sequence is selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 6,
8, 10 or 14;
b) a nucleotide sequence encoding a polypeptide comprising the amino acid
sequence set forth in SEQ ID NO:7, 9, 11 or 15;
c) a nucleotide sequence comprising at least 16 contiguous nucleotides of a
sequence of a) or b); and
d) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a, b) or c).
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Claim 34
A plant stably transformed with a DNA construct comprising a PR-1 sequence
operably linked to a promoter that drives expression of a coding sequence in a
plant cell, wherein said PR-1 sequence is selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 6,
8, 10, or 14;
b) a nucleotide sequence encoding a polypeptide comprising the amino acid
sequence set forth in SEQ ID NO: 7, 9, 11 or 15;
c) a nucleotide sequence comprising at least 16 contiguous nucleotides of a
sequence of a) or b); and
d) a nucleotide sequence that hybridizes under stringent conditions to a
sequence of a), b) or c).
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The claims include:
- DNA sequences that hybridize under stringent conditions to the promoter
sequences;
- a DNA sequence of a constitutive maize PR-1 gene promoter;
- methods for inducing the expression of a gene of interest or constitutively
expressing the gene of interest according to the promoter used;
- the nucleotide sequences of the maize PR-1 genes;
- methods for enhancing disease resistance in a plant by using the claimed
PR-1 sequences; and
- plant cells and stably transformed plants with such PR-1 genes.
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Remarks
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Related application in Europe (EP 1056862) has been
withdrawn and the application in Canada (CA 2315549 AA) is also
dead.
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