Patents on Yeast Copper-Responsive Promoter
Genentech has a granted United States patent related to inducible
transcription control sequences isolated from a yeast metallothionein gene.
These transcriptional control sequences are also known as yeast copper chelatin
promoter.
The transcriptional control sequences of the invention include the
transcriptional promoter and the metal ion regulatory region. As well, in a
vector to be replicated in suitable host, a desired gene is under the control of
a yeast chelatin promoter, a yeast chelatin transcriptional control sequence, or
a metal ion regulatory region from yeast chelatin.
The yeast chelatin transcriptional control sequence contains the chelatin
promoter, including transcription initiation and ribosome binding sites. This is
a useful promoter in its own right, but to be induced by metal ions, the metal
ion regulatory region is needed. This last region is also useful when ligated to
other, non-metallothionein promoters, which are recognized by the desired host.
An isolated DNA sequence of a yeast chelatin metal ion regulatory region is
also part of the protected invention.
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
4940661
- Earliest priority - 17 May 1984
- Filed - 29 September 1987
- Granted 10 July 1990
- Expected expiry - 29 September 2007
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Title - Metallothionein transcription control sequences and
use thereof
Claim 1
A vector comprising a selection gene, yeast origin of
replication or autonomously replicating sequence and DNA encoding a eukaryotic
polypeptide other than yeast chelatin, said DNA encoding a eukaryotic
polypeptide that is under the control of
(a) a yeast chelatin promoter,
(b) a yeast chelatin transcription control sequence, or
(c) a metal ion regulatory region of the yeast chelatin transcription
control sequence which is free of the yeast chelatin promoter, whereby the
vector is replicable in a suitable host.
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Claim 14
An isolated DNA sequence comprising a yeast chelatin metal
ion regulatory region free of the yeast chelatin promoter and free of DNA
encoding yeast chelatin.
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Genentech
Inc.
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Note: Patent information was last updated on 19 May 2006. Search terms:
"Metallothionein" in abstract and "Genentech" in applicant. Patent database:
PatentLens.
Patent on a mouse metallothionein promoter
The granted United States patent of University Patents Inc.
(now Competitive Technologies Inc.) relates to the
metal-responsive promoter/regulator sequences of the mouse
metallothionein-I gene.
The mouse metallothionein-I gene promoter of the invention is operatively
linked to a DNA sequence of interest, which is expressed in response to
variations in the concentration of metal ions in the environment. The host
organism, which stably incorporates the DNA sequence of interest, is a mammalian
host cell.
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
4579821
- Earliest priority - 23 November 1981
- Filed - 23 November 1981
- Granted - 1 April 1986
- Expired - 1 April 2003
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Title - Control of DNA sequence transcription
Claim 1
A process for subjecting the transcription of a selected DNA sequence to
external control under given environmental conditions which
comprises the steps of:
providing a selected isolated structural gene that is transcriptionally
responsive to a mouse metallothionein-I promotor/regulator DNA sequence under
the given environmental conditions; and
operatively fusing the selected structural gene with said promoter/regulator
DNA sequence.
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Claim 2
In the genetic engineering process for securing transcription and expression
of a selected isolated structural gene sequence in a mammalian host cell wherein
said selected structural gene is stably incorporated as a chromosomal or
extrachromosomal constituent of the host, the improvement
comprising the step of:
operatively fusing with said selected structural gene sequence a mouse
metallothionein-I promoter/regulator DNA sequence which is responsive to
environmental variations within the host cell in the concentration of ions of
metal.
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Claim 3
A fusion gene product, suitable for use in genetic transformation of a
mammalian host cell, said product comprising: a non mouse
metallothionein structural gene sequence to be incorporated in said host cell
operatively fused with a mouse metallothionein-I promoter/regulator DNA
sequence.
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University Patents Inc.
(now
Competitive
Technologies)
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Remarks
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The related granted European patent
EP
94428 lapsed in all European contracting states.
Related application filed in Japan (JP 7083715 B4).
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Note: Patent information was last updated on 19 May 2006. Search terms:
"transcription" in abstract and "University patents" in applicant. Patent
database: PatentLens in combination with INPADOC.
Patent on a human metallothionein promoter
The mammalian metallothionein promoter system claimed by the
University of
California
(Berkeley) in a granted United States
patent relates to a human metallothionein II transcriptional
regulatory system, which allows for regulated control of expression by
heavy metals and glucocorticoids.
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
4601978
- Earliest priority - 24 November 1982
- Filed - 24 November 1982
- Granted - 22 July 1986
- Expired - July 2003
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Title - Mammalian metallothionein promoter system
Claim 1
A DNA sequence of less than 500 base pairs, said DNA sequence
comprising the human MT-II transcriptional regulatory system
further comprising the transcription initiation sequence.
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Claim 5
A DNA construct capable of regulated expression of an inserted gene in a
mammalian host, said construct comprising an extrachromosomal
replication system recognized by a mammalian host, which replication system has
been joined to a human MT-II gene inducible regulatory system in vitro, said
construct having DNA sequence(s) defining at least one restriction site within
the transcriptional control of the regulatory region for insertion of the gene.
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Claim 10
A DNA construct useful for expression of a structural gene to produce a
polypeptide in a mammalian host, said vector comprising
(a) a replicon from bovine papilloma virus and
(b) a regulatory system from a human MT-II gene comprising
a promoter and a terminator and having at least one restriction site downstream
from the promoter in the direction of transcription.
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The claimed invention is drawn to:
- A DNA sequence encoding the human metallothionein II
transcriptional regulatory system, which includes the promoter region, the
transcriptional initiation sequence (CAP site) and the regulatory sequences
responsible for inducible transcription.
- A DNA construct where the human metallothionein II
transcriptional regulatory sequences are combined with an extra chromosomal
replication system that is recognized by a given mammalian host. The combined
system regulates the expression of an inserted gene in the mammalian host.
- A vector containing a replicon from a bovine papilloma virus and a human
metallothionein II transcriptional regulatory sequences and a
restriction site downstream of the regulatory sequences for the insertion of a
gene of interest to be expressed in a mammalian host. The bovine papilloma virus
replicon allows the vector to be replicated in the mammalian host.
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University of
California (Berkeley)
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Remarks
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Another United states patent titled " High efficiency eukaryotic
metallothionein promoter system"
(US
4511652) also expired.
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Note: Patent information was last updated on 19 May 2006. Search terms:
"metallothionein" in abstract and "University of California" in applicant.
Patent database: PatentLens.
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