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Patent Lens > Technology Landscapes > Promoters Used to Regulate Gene Expression

Specific Patent Information

Patent Number

Title, Summary of Claims and Independent Claims

Assignee

EP 637339 B1

  • Earliest priority -13 April 1992
  • Filed - 13 April 1993
  • Granted - 31 October 2001
  • Expected expiry - 13 April 2013

Title - DNA constructs and plants incorporating them

Claim 1

A chemically-inducible plant gene expression cassette comprising
    a first promoter operatively linked to the alcR regulator sequence obtainable from Aspergillus nidulans which encodes the AlcR regulator protein, and
    an inducible promoter operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene.

Claim 11

A method for controlling plant gene expression comprising
    transforming a plant cell with a chemically-inducible plant gene expression cassette which has
        a first promoter operatively linked to the alcR regulator sequence obtainable from Aspergillus nidulans which encodes the AlcR regulator protein, and
        an inducible promoter operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene.

Claim 12

A chimeric promoter comprising
    an upstream region containing a promoter regulatory sequence obtainable from the alcA gene promoter of Aspergillus nidulans and
    a downstream region containing a transcription initiation sequence, characterised in that said upstream and downstream regions are heterologous, the promoter is chemically inducible and the transcription initiation sequence is obtainable from the core promoter region of a promoter which is active in plant cells.

The claims are generally to:

  • A chemically-inducible expression cassette comprising a first promoter linked to the alcR sequence and an inducible promoter linked to a target gene. The AlcR protein activates the inducible promoter in the presence of an inducer and causes the expression of the gene.
  • A chimeric promoter comprising an upstream region with a chemically-inducible alcA gene promoter and a downstream region with a transcription initiation sequence from a core plant-expressible promoter region, in which the two regions are heterologous.
  • A method for controlling plant gene expression by transforming a plant with an expression cassette as described.

Syngenta Ltd.

US 6605754

  • Earliest priority -13 April 1992
  • Filed - 13 March 1998
  • Granted 12 August 2003
  • Expected expiry - 12 March 2018

Title - DNA constructs and plants incorporating them

Claim 1

A plant cell which contains stably integrated into its genome a gene expression cassette, said gene expression cassette comprising
    a first promoter operatively linked to a sequence comprising an alcR coding sequence from Aspergillus nidulans and which encodes an ALCR regulator protein, and
    an inducible promoter from an ALCR-activatable gene, which gene is the alcA gene from Aspergillus nidulans, operatively linked to a target gene, said inducible promoter being activated by the ALCR regulator protein in the presence of an alcohol and/or ketone inducer, so that application of a sufficient amount of a suitable inducer causes expression of the target gene.

Claim 7

A method for controlling plant gene expression comprising
    transforming a plant cell with a chemically-inducible gene expression cassette which has
        a first promoter operatively linked to a sequence comprising an alcR coding sequence from Aspergillus nidulans, and which encodes an ALCR regulator protein, and
        an inducible promoter from an ALCR-activatable gene, which gene is the alcA gene from Aspergillus nidulans, operatively linked to a target gene, said inducible promoter being activated by the ALCR regulator protein in the presence of an alcohol and/or ketone inducer, so that application of a sufficient amount of a suitable inducer causes expression of the target gene.

Claim 8

A plant cell containing a chimeric promoter operatively linked to a target gene, said chimeric promoter comprising
    an upstream region containing a promoter from an ALCR-activatable alcA gene from Aspergillus nidulans and     a downstream region containing a transcription initiation sequence, wherein the upstream region and the downstream region are heterologous and the chimeric promoter is inducible by an alcohol and/or ketone, so that application of a sufficient amount of a suitable inducer, in the presence of the ALCR regulator protein encoded by the alcR gene from Aspergillus nidulans, causes expression of the target gene.

The claims of this US patent is genearlly directed to:

  • Plant cells containing a chemically-inducible plant gene expression cassette or chimeric promoter operatively linked to a target gene as described in EP 637339 B1.
  • A method for controlling plant gene expression by transforming a plant cell with the chemically-inducible gene expression cassette as described so that gene expression is controlled by an alcohol and/or ketone inducer.

Remarks

The application in Australia (AU 39019/93 A1) has lapsed.

Note: Patent information was last updated on 11 May 2006. Search terms: "promoter" in abstract and "Syngenta" in application. Patent database: PatentLens in combination with INPADOC.

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