Specific Patent Information
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Patent Number
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Title, Summary of Claims and Independent Claims
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Assignee
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EP
637339 B1
- Earliest priority -13 April 1992
- Filed - 13 April 1993
- Granted - 31 October 2001
- Expected expiry - 13 April 2013
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Title - DNA constructs and plants incorporating them
Claim 1
A chemically-inducible plant gene expression cassette
comprising
a first promoter operatively linked to the alcR regulator sequence
obtainable from Aspergillus nidulans which encodes the AlcR regulator
protein, and
an inducible promoter operatively linked to a target gene, the inducible
promoter being activated by the regulator protein in the presence of an
effective exogenous inducer whereby application of the inducer causes expression
of the target gene.
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Claim 11
A method for controlling plant gene expression comprising
transforming a plant cell with a chemically-inducible plant gene expression
cassette which has
a first promoter operatively linked to the alcR regulator sequence
obtainable from Aspergillus nidulans which encodes the AlcR regulator
protein, and
an inducible promoter operatively linked to a target gene, the inducible
promoter being activated by the regulator protein in the presence of an
effective exogenous inducer whereby application of the inducer causes expression
of the target gene.
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Claim 12
A chimeric promoter comprising
an upstream region containing a promoter regulatory sequence obtainable from
the alcA gene promoter of Aspergillus nidulans and
a downstream region containing a transcription initiation sequence,
characterised in that said upstream and downstream regions are heterologous, the
promoter is chemically inducible and the transcription initiation sequence is
obtainable from the core promoter region of a promoter which is active in plant
cells.
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The claims are generally to:
- A chemically-inducible expression cassette comprising a first promoter
linked to the alcR sequence and an inducible promoter linked to a
target gene. The AlcR protein activates the inducible promoter in the presence
of an inducer and causes the expression of the gene.
- A chimeric promoter comprising an upstream region with a
chemically-inducible alcA gene promoter and a downstream region with a
transcription initiation sequence from a core plant-expressible promoter region,
in which the two regions are heterologous.
- A method for controlling plant gene expression by transforming a plant with
an expression cassette as described.
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Syngenta
Ltd.
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US
6605754
- Earliest priority -13 April 1992
- Filed - 13 March 1998
- Granted 12 August 2003
- Expected expiry - 12 March 2018
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Title - DNA constructs and plants incorporating them
Claim 1
A plant cell which contains stably integrated into its genome a gene
expression cassette, said gene expression cassette comprising
a first promoter operatively linked to a sequence comprising an alcR coding
sequence from Aspergillus nidulans and which encodes an ALCR regulator
protein, and
an inducible promoter from an ALCR-activatable gene, which gene is the alcA
gene from Aspergillus nidulans, operatively linked to a target gene,
said inducible promoter being activated by the ALCR regulator protein in the
presence of an alcohol and/or ketone inducer, so that application of a
sufficient amount of a suitable inducer causes expression of the target gene.
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Claim 7
A method for controlling plant gene expression comprising
transforming a plant cell with a chemically-inducible gene expression
cassette which has
a first promoter operatively linked to a sequence comprising an alcR
coding sequence from Aspergillus nidulans, and which encodes an ALCR
regulator protein, and
an inducible promoter from an ALCR-activatable gene, which gene is the
alcA gene from Aspergillus nidulans, operatively linked to a target
gene, said inducible promoter being activated by the ALCR regulator protein in
the presence of an alcohol and/or ketone inducer, so that application of a
sufficient amount of a suitable inducer causes expression of the target gene.
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Claim 8
A plant cell containing a chimeric promoter operatively linked to a target
gene, said chimeric promoter comprising
an upstream region containing a promoter from an ALCR-activatable alcA gene
from Aspergillus nidulans and a downstream region containing a
transcription initiation sequence, wherein the upstream region and the
downstream region are heterologous and the chimeric promoter is inducible by an
alcohol and/or ketone, so that application of a sufficient amount of a suitable
inducer, in the presence of the ALCR regulator protein encoded by the alcR gene
from Aspergillus nidulans, causes expression of the target gene.
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The claims of this US patent is genearlly directed to:
- Plant cells containing a chemically-inducible plant gene expression cassette
or chimeric promoter operatively linked to a target gene as described in
EP
637339 B1.
- A method for controlling plant gene expression by transforming a plant cell
with the chemically-inducible gene expression cassette as described so that gene
expression is controlled by an alcohol and/or ketone inducer.
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Remarks
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The application in Australia (AU 39019/93 A1) has lapsed.
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Note: Patent information was last updated on 11 May 2006. Search terms:
"promoter" in abstract and "Syngenta" in application. Patent database:
PatentLens in combination with INPADOC.
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time it was collated. New patents and patent applications, altered
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