Other plant actin promoters
Rice actin promoters have been shown to be strong in directing gene
expression in graminaceous monocotyledonous plants. However, their function in
certain non-graminaceous monocots are variable suggesting that the activity of
actin promoters may be limited to closely related species. Therefore, Researches
were also carried out to identify actin promoters from other plant species. So
far, patents have been issued and patent applications have been filed on actin
promoters from the plant species including banana, melon, maize and
Arabidopsis.
Queensland University of Technology filed patent applications in the United
States and Australia on an actin promoter from banana and its use.
Exelixis Plant Sciences, Inc. obtained patents in United States and
Australia on a melon actin promoter.
Monsanto owns two United States patents and one Australian patent claiming
Arabidopsis actin promoters and their use as part of the invention. In addition,
Monsanto also filed patent applications on actin regulatory elements from rice
and maize in the United States and Europe.
Exelixis Plant Sciences, Inc. patent family
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Patent/Application No
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6642438
- Earliest priority - 19 Mar 1999
- Filed - 17 Mar 2000
- Granted - 04 Nov 2003
- Expected expiry - 17 Mar 2020
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Title - Melon promoters for expression of transgenes in
plants
Claim 1
An isolated nucleic acid molecule comprising a promoter, wherein the promoter
comprises the nucleic acid sequence presented as
SEQ
ID NO:4.
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Claim 2
An isolated nucleic acid molecule comprising a promoter, wherein the promoter
consists of a portion of the nucleic acid sequence presented as
SEQ
ID NO:4 that, when operably linked to a protein-encoding polynucleotide
sequence, directs expression of the protein in a plant cell.
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The claims are directed to a promoter isolated from melon (Cucumis melo) and
its use including:
- An isolated nucleic acid molecule comprising a promoter
- A method for producing a transgenic plant with constitutive expression of
protein encoding polynucleotide sequence under the control of the promoter
(Claim 7).
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Exelixis Plant Sciences, Inc.
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AU
782602 B2
- Earliest priority - 19 Mar 1999
- Filed - 17 Mar 2000
- Granted - 04 Nov 2003
- Expected expiry - 17 Mar 2020
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Title - Banana and melon promoters for expression of transgenes in plants
The independent claims of this Australian patent are the same as that in
US
6642438.
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Remarks
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Patent applications were also filed in Europe (EP 1165755
A1), Canada (CA 2365259) and Japan (JP
2002539779 T2).
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Note: Patent information was last updated on 9 November 2006. Search terms:
"plant
actin promoter" in abstract. Patent database: PatentLens in combination with
INPADOC.
Monsanto patent family on Arabidopsis actin promoters
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Patent/Application No
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6462258
- Earliest priority - 16 Dec 1999
- Filed - 15 Dec 2000
- Granted - 15 Dec 2000
- Expected expiry - 2 Mar 2021 (adjusted)
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Title - Plant expression constructs
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Claim 1
A DNA construct comprising: a first expression cassette, a second expression
cassette, and a third expression cassette, wherein said first expression
cassette in operable linkage comprises
(i) a Figwort mosaic virus 35S promoter;
(ii) a heterologous 5' untranslated leader;
(iii) a chloroplast transit peptide DNA sequence;
(iv) a DNA sequence encoding a glyphosate tolerant EPSPS; and
(v) a transcriptional terminator; and
said second expression cassette comprising in operable linkage
(a) an Arabidopsis Elongation factor 1α promoter with homologous EF1α
intron;
(b) a chloroplast transit peptide DNA sequence;
(d) a DNA sequence encoding a glyphosate tolerant EPSPS; and
(e) a transcriptional terminator; and
said third expression cassette comprising in operable linkage
(I) an Arabidopsis Act2 promoter with homologous Act2 intron;
(II) a chloroplast transit peptide DNA sequence;
(III) a DNA sequence encoding a glyphosate tolerant EPSPS; and
(IV) a transcriptional terminator.
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MONSANTO TECHNOLOGY LLC
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US
6919495
- Earliest priority - 16 Dec 1999
- Filed - 01 May 2003
- Granted - 19 Jul 2005
- Expected expiry - 14 Jun 2023 (adjusted)
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Title - Chimeric cauliflower mosaic virus 35S-arabidopsis
actin 8 promoters and methods of using them
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Claim 1
A DNA construct comprising a chimeric promoter DNA sequence comprising
SEQ
ID NO:29; a structural DNA sequence; and a 3' non-translated region that
functions in plants to cause the addition of polyadenylated nucleotides to the
3' end of the RNA sequence; wherein the structural DNA sequence is operably
linked to the chimeric promoter and the 3' non-translated region, and the
chimeric promoter DNA sequence is heterologous with respect to the structural
DNA sequence.
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Claim 7
A DNA construct comprising a chimeric promoter DNA sequence comprising
SEQ
ID NO:29; an aroA:CP4 structural DNA sequence; and a 3' non-translated
region that functions in plants to cause the addition of polyadenylated
nucleotides to the 3' end of the RNA sequence; wherein the structural DNA
sequence is operably linked to the chimeric promoter and the 3' non-translated
region, and the chimeric promoter DNA sequence is heterologous with respect to
the structural DNA sequence.
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Claim 8
A method of expressing a structural DNA sequence in a plant, the method
comprising:
(1) providing a DNA construct comprising a promoter that is functional in
a plant cell, the promoter comprising
SEQ
ID NO:29; a structural DNA sequence; and a 3' non-translated region that
functions to cause the addition of polyadenylated nucleotides to the 3' end of
the RNA sequence; wherein the structural DNA sequence is operably linked to the
promoter and the 3' non-translated region, and the promoter is heterologous with
respect to the structural DNA sequence;
(2) introducing the DNA construct into a plant cell;
(3) regenerating the plant cell to produce the plant and
(4) screening seedlings to identify plants that express the structural
DNA sequence.
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AU
782697 B2
- Earliest priority - 16 Dec 1999 (US)
- Filed - 12 Dec 2000
- Granted - 18 Aug 2005
- Expected expiry - 12 Dec 2020
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Title - DNA constructs for expression of heterologous
polypeptides in plants
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Claim 1
A hybrid promoter DNA sequence comprising at least one cis element
from a caulimovirus promoter operably linked to at least one cis
element from an Arabidopsis promoter selected from an Act2 promoter,
Act8 promoter or EF1α promoter.
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Remarks
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Related patent applications were also filed in Europe (EP 1632576
A2), Canada (CA 2394984), China (CN
1434868), Japan (JP 2003516753), Brazil (BR
200016460) and Indonesia (ID 28665).
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Note: Patent information was last updated on 9 November 2006. Search terms:
"actin" in title. Patent database: PatentLens in combination with INPADOC.
Monsanto patent family on on actin regulatory elements from rice and maize
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Patent/Application No
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Title, Independent Claims and Summary of Claims
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Assignee
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US
2006/162010 A1
- Earliest priority - 25 Sep 2003
- Filed - 24 Sep 2004
- Granted - pending
- Expected expiry - N/A
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Title - Actin regulatory elements for use in plants
Claim 1
An isolated polynucleotide molecule having gene regulatory activity and
comprising a polynucleotide sequence selected from the group consisting of SEQ
ID NO: 1-7.
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Claim 4
A DNA construct comprising an isolated polynucleotide molecule having gene
regulatory activity and comprising a polynucleotide sequence selected from the
group consisting of SEQ ID NO: 1-7, wherein said isolated polynucleotide
molecule is operably linked to a transcribable polynucleotide molecule.
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Claim 15
A method of inhibiting weed growth in a field of transgenic glyphosate
tolerant crop plants comprising: i) planting the transgenic plants transformed
with an expression cassette comprising (a) an isolated polynucleotide molecule
having gene regulatory activity and comprising a polynucleotide sequence
selected from the group consisting of SEQ ID NO: 1-7 and operably linked to a
DNA molecule encoding a glyphosate tolerance gene and ii) applying glyphosate to
the field at an application rate that inhibits the growth of weeds, wherein the
growth and yield of the transgenic crop plant is not substantially affected by
the glyphosate application.
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MONSANTO TECHNOLOGY LLC
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Remarks
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Related European patent application (EP 1664311 A2) was also
filed. Its PCT application is
WO
2005/030968.
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Note: Patent information was last updated on 9 November 2006. Search terms:
"plant actin promoter" in abstract. Patent database: PatentLens in combination
with INPADOC.
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