Ubiquitin promoters from rice and sugarcane
Apart from patents on the ubiquitin regulatory system from maize, there are
also patents specifically claiming ubiquitin promoters from rice and sugarcane.
The Board of Supervisors of Louisiana State University and Agricultural and
Mechanical College holds a United States patent
(US
6528701) on rice ubiquitin promoters. This patent claims a rice
ubiquitin promoter and a method for using it in controlling gene expression in
plants. It shows in the description that the rice ubiquitin promoter is stronger
than the maize ubiquitin promoter in driving gene expression in rice.
United States of America as represented by the Secretory of Agriculture &
University of Hawaii has three United States patents
(US
6706948,
US
6686513 and
US
6638766) on sugarcane ubiquitin promoters (UBI4 and UBI9).
rice ubiquitin promoters
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Title, Independent Claims and
Summary of Claims
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Assignee
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US
6528701
- Earliest priority - 29 Feb 2000
- Filed - 29 Feb 2000
- Granted - 04 Mar 2003
- Expected expiry - 29 Feb 2020
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Title - Rice ubiquitin-derived promoters
Claim 1
An isolated nucleotide sequence comprising a rice ubiquitin promoter capable
of controlling constitutive expression of a nucleic acid encoding a polypeptide,
wherein said nucleotide sequence comprises at least a portion of
SEQ
ID NO:3 which is upstream of position 2785 and wherein said portion retains
promoter activity.
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The claims of this patent are generally drawn to:
- An isolated nucleotide sequence comprising a rice ubiquitin promoter
(upstream of position 2785 of SEQ ID NO:3) , and
- A method of expressing a heterologous nucleic acid sequence encoding a
polypeptide in a plant or in its progeny under the control of the rice ubiquitin
promoter (claim 6).
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Board of Supervisors of Louisiana State University and
Agricultural and Mechanical College
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Note: Patent information was last updated on 31 October 2006. Search terms:
"Ubiquitin promoter" in abstract. Patent database: PatentLens in combination
with INPADOC.
Patents and applications on sugarcane ubiquitine promoters
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Patent/application number
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Title, Independent Claims and
Summary of Claims
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Assignee
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US
6706948
- Earliest priority - 19 Mar 1998
- Filed - 17 Mar 1999
- Granted - 16 Mar 2004
- Expected expiry - 17 Mar 2019
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Title - Sugarcane UBI9 gene promoter and methods of use
thereof
Claim 1
A substantially purified nucleic acid sequence comprising a nucleotide
sequence selected from the group consisting of
SEQ
ID NO:7, the complement of
SEQ
ID NO:7,
SEQ
ID NO:10, and the complement of
SEQ
ID NO:10.
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Claim 4
A substantially purified nucleic acid sequence comprising the HindIII/XbaI
fragment isolated from plasmid pubi9-GUS contained in Escherichia coli cells
deposited as NRRLB-30116.
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Claim 5
A transgenic plant cell comprising a nucleic acid sequence comprising a
double-stranded nucleotide sequence listed as
SEQ
ID NO:10, wherein said nucleotide sequence is operably linked to a nucleic
acid sequence of interest.
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Claim 6
A method for expressing a nucleic acid sequence of interest in a plant cell,
comprising:
a) providing:
i) a plant cell;
ii) a nucleic acid sequence of interest; and
iii) a nucleotide sequence selected from the group consisting of
SEQ
ID NO:10, and the complement of
SEQ
ID NO:10;
b) operably linking said nucleic acid sequence of interest to said
nucleotide sequence to produce a transgene; and
c) introducing said transgene into said plant cell to produce a
transgenic plant cell under conditions such that said nucleic acid sequence of
interest is expressed in said transgenic plant cell.
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Claim 7
A method for expressing a nucleic acid sequence of interest in a plant cell,
comprising:
a) providing:
i) a plant cell;
ii) a nucleic acid sequence of interest; and
iii) a promoter comprising
SEQ
ID NO:10;
b) operably linking said nucleic acid sequence of interest to said
promoter to produce a transgene;
c) introducing said transgene into said plant cell to produce a
transgenic plant cell under conditions such that said nucleic acid sequence of
interest is expressed in said transgenic plant cell, and
d) identifying said transgenic plant cell.
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The claims of this patent are directed to:
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United States of America as represented by the
Secretory of Agriculture & University of Hawaii
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US
6686513
- Earliest priority - 19 Mar 1998
- Filed - 20 Oct 2000
- Granted - 03 Feb 2004
- Expected expiry - 17 Mar 2019
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Title - Sugarcane ubi9 gene promoter sequence and methods of
use thereof
Claim 1
A transgenic plant cell comprising a promoter comprising
SEQ
ID NO:10, wherein said promoter is operably linked to a nucleic acid
sequence of interest, and said plant cell is selected from sugar cane, tobacco,
sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic.
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Claim 6
A method for expressing a nucleic acid sequence of interest in a plant cell
selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato,
soybean, banana, and garlic, comprising:
a) providing:
i) said plant cell;
ii) a nucleic acid sequence of interest; and
iii) a promoter comprising
SEQ
ID NO: 10;
b) operably linking said nucleic acid sequence of interest to said
promoter to produce a transgene; and
c) introducing said transgene into said plant cell to produce a
transgenic plant cell under conditions such that said nucleic acid sequence of
interest is expressed in said transgenic plant cell.
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This patent is a Continuation in part of
US
6706948.
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US
6638766
- Earliest priority - 19 Mar 1998
- Filed - 24 May 2001
- Granted - 28 Oct 2003
- Expected expiry - 4 Jun 2019 (adjusted)
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Title - Promoter of the sugarcane UBI4 gene
Claim 1
A substantially purified nucleic acid sequence comprising the nucleotide
sequence selected from the group consisting of SEQ ID NO:7 and the complement
thereof.
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Claim 4
A transgenic plant cell comprising a nucleic acid sequence comprising the
double-stranded nucleotide sequence listed as
SEQ
ID NO:7, wherein said nucleotide sequence is operably linked to a nucleic
acid sequence of interest.
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This patent is a Continuation of
US
6706948.
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Remarks
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Related patent applications were also filed in Australia (AU 3192799
A1), Europe (EP 1063880 A1), Canada
(CA 2324520) and Israel (IL 138580). However,
the applications in Australia, Europe and Canada all lapsed.
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Note: Patent information was last updated on 31 October 2006. Search terms:
"polyubiquitin" in abstract. Patent database: PatentLens in combination with
INPADOC.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
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