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Information About pCAMBIA Vectors:

There are two types of pCAMBIA plasmids: legacy pCAMBIA plasmids and pCAMBIA GUSPlus plasmids.

GUSPlus™ is a new reporter gene for use in molecular biology. There are GUSPlus vectors for checking transformations and screening transformants, and special vectors for use with TransBacter strains. The GUSPlus gene was originally isolated from Staphylococcus sp. but has been extensively altered by codon optimisation for expression in plants.

When you order pCAMBIA GUSPlus plasmids you can either order:

  1. The pCAMBIA GUSPlus kit which contains pCAMBIA1105.1, pCAMBIA1305.1, pCAMBIA1305.2, pCAMBIA0305.1 and pCAMBIA0305.2
  2. or individual plasmid pCAMBIA5105 

If you wish to discuss or query any of CAMBIA's materials please login to the Bioforge

Frequently Asked Questions - pCAMBIA Vectors

General information on pCAMBIA plasmids

What concentration of antibiotics should I use?

What concentration of plant selection antibiotic should I use?

What versions of the gus genes are used in the pCAMBIA vectors?

Which vector should I use?

Which plasmids have the hexa-His tag appended to the reporter gene?

Which version of gfp is in the vectors?

I’m having trouble with the gfp-containing vectors.

I’m worried about the 35S promoter interfering with my promoter study.

Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?

Will I have full freedom to use the pCAMBIA vectors?

What if I use a pCAMBIA vector to make a new vector?

Can I modify pCAMBIA vectors?

To order GUSPlus and other newer pCAMBIA vectors:

Resources:

List of CAMBIA Materials