Can I modify pCAMBIA vectors?

General information on pCAMBIA plasmids

What concentration of antibiotics should I use?

What concentration of plant selection antibiotic should I use?

What versions of the gus genes are used in the pCAMBIA vectors?

Which vector should I use?

Which plasmids have the hexa-His tag appended to the reporter gene?

Which version of gfp is in the vectors?

I’m having trouble with the gfp-containing vectors.

I’m worried about the 35S promoter interfering with my promoter study.

Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?

Will I have full freedom to use the pCAMBIA vectors?

What if I use a pCAMBIA vector to make a new vector?

Can I modify pCAMBIA vectors?

Please do feel free to modify pCAMBIA vectors, and share the modifications with others via the BioForge (http://bioforge.net/forge/forum.jspa?forumID=31). Others may be interested in using the same types of plasmids you've made, and sharing will save everyone work. Someone may do the same for you later.

If you plan to distribute vectors you've derived from pCAMBIA vectors, please ask the recipients to send the pCAMBIA vector Materials Transfer Agreement form to us and provide a copy to you as well. If you'd like us to distribute the vectors, we can do so under a BiOS-compliant MTA or license (www.bioslicense.net) and would be glad to do so.

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