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I’m having trouble with the gfp-containing vectors.
Researchers at CAMBIA have found that the mGFP5 protein and its GUSA fusion proteins are not particularly bright. Confocal microscopy is generally needed to get reasonable results. Much better results have been achieved with the egfp gene. Constructs analogous to pCAMBIA1302, 1303 & 1304 using egfp in the place of mgfp5 gave far better results in our hands in rice and Arabidopsis. We are not authorised to distribute egfp-containing vectors, however. We can supply protocols on how we made these vectors. For a license to egfp, if you are in a jurisdiction in which you would require it for freedom to operate, you should contact Clontech.
General information on pCAMBIA plasmids
What concentration of antibiotics should I use?
What concentration of plant selection antibiotic should I use?
What versions of the gus genes are used in the pCAMBIA vectors?
Which plasmids have the hexa-His tag appended to the reporter gene?
Which version of gfp is in the vectors?
I’m having trouble with the gfp-containing vectors.
I’m worried about the 35S promoter interfering with my promoter study.
Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?
Will I have full freedom to use the pCAMBIA vectors?
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