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What concentration of plant selection antibiotic should I use?
|
pCAMBIA series |
1xxx |
2xxx |
3xxx |
|---|---|---|---|
|
Resistance Gene |
hph (From a Klebsiella species, via E.coli) |
nptII/aph(3')II from Tn5 |
bar/pat from Streptomyces hygroscopicus |
|
Transformed plant selection antibiotic |
Hygromycin B |
Geneticin (G418) |
Bialaphos |
|
Concentration in Arabidopsis |
50μg/mL in germination medium |
25μg/mL |
BASTA 100μg/mL (for spraying on plants) |
|
Concentration in Rice |
50μg/mL in 2N6 then in RGH6 |
Not tested |
Bialaphos 3μg/mL (for selection on medium) |
|
Concentration in Tobacco |
50μg/mL in RMOP then in MST |
Not tested |
Bialaphos 1μg/mL (for selection on medium) |
|
Concentration in other plants |
* |
* |
* |
*Please send us information based on your results using pCAMBIAs in other plants and we will add the information to this table.
General information on pCAMBIA plasmids
What concentration of antibiotics should I use?
What concentration of plant selection antibiotic should I use?
What versions of the gus genes are used in the pCAMBIA vectors?
Which plasmids have the hexa-His tag appended to the reporter gene?
Which version of gfp is in the vectors?
I’m having trouble with the gfp-containing vectors.
I’m worried about the 35S promoter interfering with my promoter study.
Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?
Will I have full freedom to use the pCAMBIA vectors?
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