Main Menu

What concentration of plant selection antibiotic should I use?

pCAMBIA series

1xxx

2xxx

3xxx

Resistance Gene

hph (From a Klebsiella species, via E.coli)

nptII/aph(3')II from Tn5

bar/pat from Streptomyces hygroscopicus

Transformed plant selection antibiotic

Hygromycin B

Geneticin (G418)

Bialaphos
BASTA

Concentration in Arabidopsis

50μg/mL in germination medium

25μg/mL

BASTA 100μg/mL (for spraying on plants)

Concentration in Rice

50μg/mL in 2N6 then in RGH6

Not tested

Bialaphos 3μg/mL (for selection on medium)

Concentration in Tobacco

50μg/mL in RMOP then in MST

Not tested

Bialaphos 1μg/mL (for selection on medium)
BASTA 100μg/mL (for spraying on plants)

Concentration in other plants

*

*

*

*Please send us information based on your results using pCAMBIAs in other plants and we will add the information to this table.