General information on pCAMBIA plasmids
What concentration of antibiotics should I use?
What concentration of plant selection antibiotic should I use?
What versions of the gus genes are used in the pCAMBIA vectors?
Which plasmids have the hexa-His tag appended to the reporter gene?
Which version of gfp is in the vectors?
I’m having trouble with the gfp-containing vectors.
I’m worried about the 35S promoter interfering with my promoter study.
Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?
Will I have full freedom to use the pCAMBIA vectors?
What concentration of plant selection antibiotic should I use?
|
pCAMBIA series |
1xxx |
2xxx |
3xxx |
|---|---|---|---|
|
Resistance Gene |
hph (From a Klebsiella species, via E.coli) |
nptII/aph(3')II from Tn5 |
bar/pat from Streptomyces hygroscopicus |
|
Transformed plant selection antibiotic |
Hygromycin B |
Geneticin (G418) |
Bialaphos |
|
Concentration in Arabidopsis |
50μg/mL in germination medium |
25μg/mL |
BASTA 100μg/mL (for spraying on plants) |
|
Concentration in Rice |
50μg/mL in 2N6 then in RGH6 |
Not tested |
Bialaphos 3μg/mL (for selection on medium) |
|
Concentration in Tobacco |
50μg/mL in RMOP then in MST |
Not tested |
Bialaphos 1μg/mL (for selection on medium) |
|
Concentration in other plants |
* |
* |
* |
*Please send us information based on your results using pCAMBIAs in other plants and we will add the information to this table.
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