General information on pCAMBIA plasmids
What concentration of antibiotics should I use?
What concentration of plant selection antibiotic should I use?
What versions of the gus genes are used in the pCAMBIA vectors?
Which plasmids have the hexa-His tag appended to the reporter gene?
Which version of gfp is in the vectors?
I’m having trouble with the gfp-containing vectors.
I’m worried about the 35S promoter interfering with my promoter study.
Where can I get pCAMBIA3200, pCAMBIA3201, pCAMBIA3300 & pCAMBIA3301?
Will I have full freedom to use the pCAMBIA vectors?
What concentration of antibiotics should I use?
pCAMBIAx1xx vectors – spectinomycin/streptomycin resistance (aadA/aminoglycoside-3’’-adenylyltransferase) in bacteria: use 50μg/mL in E.coli & A.tumefaciens
pCAMBIAx2xx vectors – chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli & A.tumefaciens.
pCAMBIAx3xx vectors – kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from Enterococcus faecalis pJH1) in bacteria: use 50μg/mL in E.coli & A.tumefaciens.
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