DRAFT
CambiaLabs > Projects > DArT (Diversity Arrays Technology)

Principles of DArT

Principle_DArT

  • What is a DArT marker?
    A DNA segment, present or absent in a defined genomic representation, depending on the individual genotype.
  • What is a genomic representation?
    A specific fraction of the genome, produced in a reproducible manner by applying to the genome a complexity reduction method. Genomic representations used in DArT usually comprise 0.1 to 10% of the genome depending on the initial complexity of the organism. There are many possible methods to produce a genomic representation of the appropriate complexity.
  • What is the molecular basis of polymorphism?
    Any change that causes the defined segment to be scored as present or absent in the representation, for example: SNP or differential methylation in restriction sites used to generate the representation, insertion or deletion in the genomic sequence corresponding to the marker (including transposons).
  • How to score?
    By hybridisation to a probe homologous to the marker. Many different platforms can be used for scoring. At this stage a DNA-microarray platform is being used by most licensees, but other platforms can be contemplated. CAMBIA encourages improvement of the technology for multiple platforms by making it available via the non-exclusive Genetic Resource Indexing Technology BiOS License.
  • Where are the markers in the genome?
    In principle they should be anywhere; data available in rice and barley suggest this is correct.
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