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Patent Lens > Technology Landscapes > Resistance to Phosphinothricin

1. Dominant bar gene patents

Patents originally assigned to Plant Genetic Systems NV and Biogen NV. These patents are now part of the dominant patent family in the Bayer Crop Science portfolio.

Granted actual claims.

EP 242236 B2

Claim 1

Process for controlling the action in plant cells and plants comprising such cells of a glutamine synthetase inhibitor when the former are contacted with the latter, which comprises causing the stable integration in the genomic DNA of said plant cells of a heterologous DNA including a promoter recognized by polymerases of said plant cells and a foreign nucleotide sequence capable of being expressed in the form of a protein in said plant cells and plants, under the control of said promoter, and wherein said protein has an enzymatic activity capable of causing inactivation or neutralization of said glutamine synthetase inhibitor.

Claim 2

Process according to claim 1, wherein the heterologous DNA fragment comprises a foreign nucleotide sequence coding for a polypeptide having an acetyl transferase activity particularly PPT acetyltransferase activity with respect to said glutamine synthetase inhibitor.

Claim 3

Process according to claims 1 or 2, wherein the foreign nucleotide sequence is derived from the genome of an antibiotic-producing Streptomyces strain or is a nucleotide sequence encoding the same activity.

Claim 7

A process for producing a plant or reproduction material of said plant including a heterologous genetic material stably integrated therein and capable of being expressed in said plants or reproduction material in the form of a protein capable of inactivating of neutralizing the activity of a glutamine sy nthetase inhibitor, which process comprises transforming cells or tissue of said plants with a DNA recombinant containing a heterologous DNA including a foreign nucleotide sequence encoding said protein as well as the regulatory elements selected among those which are capable of causing the stable integration of said heterologous DNA in said plant cells or tissue and of enabling the expression of said foreign nucleotide sequence in said plant cells or plant tissue, regenerating plants or reproduction material of said plants or both from the plants cells or tissue transformed with said heterologous DNA and, optionally biologically replicating said last mentioned plants or reproduction material or both.

Claim 18

Process for selectively protecting the culture of a plant species and selectively destroying weeds which comprises the steps of treating the field with a herbicide consisting of a glutamine synthetase inhibitor, wherein the cells of the plant species contain in their genome a foreign nucleotide sequence encoding a protein having an enzymatic activity capable of neutralizing or inactivating said glutamine synthetase inhibitor under the control of a promoter recognized by the polymerases of the cells of said plant.

Claim 23

Process for selectively protecting a plant species in a field against fungal diseases comprising the steps of treating said field with a herbicide consisting of a glutamine synthetase inhibitor, wherein the plant species contains in the genome of its cells a heterologous DNA including a promoter recognized by the polymerases of said cell and a foreign nucleotide sequence encoding a protein having an enzymatic activity capable of neutralizing or inactivating said glutamine synthetase inhibitor under the control of said promoter.

Abandoned Claims

Claim 18

Seeds, non biologically transformed which possess, stably integrated in the genome of their cells, a heterologous DNA containing a promoter recognized by the polymerases of said seeds and a foreign nucleotide sequence encoding a protein having a non-variety-specific enzymatic activity capable of inactivating or neutralizing a glutamine synthetase inhibitor under the control of said promoter.

Claim 19

Seeds according to claim 18, which are capable of germinating into a plant capable of producing seeds having a non-variety-specific enzymatic activity capable of inactivating or neutralizing glutamine synthetase inhibitor.

Claim 20

Seeds according to claim 18 or 19, which are transformed by the process of any one of claims 7 to 13.

Claim 21

Plant, non biologically transformed, which possesses, stably integrated in the genome of its cells, a foreign DNA nucleotide sequence encoding a protein having a non-variety-specific enzymatic activity capable of neutralizing or inactivating a glutamine synthetase inhibitor under the control of a promoter recognized by the polymerases of said cells.

Claim 22

Plant according to claim 21, which is capable of producing seeds having a non-variety-specific enzymatic activity capable if inactivating or neutralizing a glutamine synthetase inhibitor.

Claim 23

Plants according to claim 21 or 22, which are transformed by the process if any of claims 7 to 13.

US 5561236

Claim 1

A plant cell having a heterologous DNA stably integrated into its genome; said DNA comprising a heterologous DNA fragment encoding a protein having an acetyl transferase activity which inactivates a glutamine synthetase inhibitor in said cell.

Claim 2

The cell of claim 1 wherein said DNA fragment encodes a polypeptide having a phosphinothricin acetyl transferase activity with respect to Bialaphos or phosphinothricin.

Claim 8

A plant which consists of the cells of claim 1 and which is susceptible to infection and transformation by Agrobacterium and capable of regeneration thereafter.

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