Brassica - Patent Assigned to AgrEvo Canada Inc. (now Bayer Crop Science)

A method for producing a stably transformed Brassica embryogenic microspore, capable of leading to a non-chimeric transformed haploid or doubled haploid embryo which develops into a fertile homozygous Brassica plant within one generation, said process comprising the following steps:
a. infecting an embryogenic microspore with Agrobacteria, which contain a plasmid carrying a gene of interest under regulatory control of initiation and termination signals bordered by at least one T-DNA border, and
b. washing out and killing the Agrobacteria after co-cultivation using mucolytic enzymes, thereby producing a stably transformed Brassica embryogenic microspore.

A method for producing a non-chimeric Brassica plant, containing a foreign DNA stably incorporated into its genome, said method comprising:
a. co-cultivating a Brassica embryogenic microspore with Agrobacteria which contains a plasmid carrying a gene of interest under regulatory control of initiation and termination signals;
b. washing out and killing the Agrobacteria after co-cultivation using mucolytic enzymes; and
c. regenerating a non-chimeric haploid or doubled haploid Brassica embryo from said microspore, wherein the embryo contains said gene of interest stably integrated into its genome, thereby producing a non-chimeric Brassica plant.