Soybean - Patent applications filed by Monsanto

A method of preparing a germline-transformed soybean plant using Agrobacterium mediation, the method comprising: A) initiating the germination of a soybean seed;
B) isolating the embryonic axis from the soybean seed to prepare an explant;
C) wounding the explant;
D) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct including a selectable marker gene under conditions in which the heterologous genetic construct is transferred into at least one cell in the explant;
E) culturing the explant in the presence of a selection agent in a manner capable of identifying soybean cells of the explant to which the heterologous genetic construct has been transferred;
F) inducing formation of one or more shoots from the explant, the shoot comprising germline transformed cells;
G) cultivating the shoot into a whole fertile mature soybean plant.