Soybean
Summary
The University of
Toledo, Monsanto, Pioneer Hi-Bred
and University of Mississippi collectively have several patents
granted in the United States, Canada, Australia and Europe which are directed to
Agrobacterium transformation of soybean (Glycine max).
The University of Toledo has both an Australian and a United States patent which claim a non-tissue culture process to transform meristematic or mesocotyl cells of a soybean seed. An Agrobacterium strain of any species is used for the transformation and can contain either an armed (with tumor-causing genes) or a disarmed vector. A related European patent additionally claims a method for transforming beans (Phaseolus vulgaris).
In the United States patents granted to Monsanto, a cotyledon from a soybean seedling or an embryonic axis are used for transformation with Agrobacterium containing a disarmed vector. Additionally they claim the insertion of a gene encoding for neomycin phosphotransferase II (nptII) and a gene encoding EPSPS. The first gene confers resistance to kanamycin and neomycin among other antibiotics, and the later confers resistance to the herbicide glyphosate.
A United States patent (US 6384301 B1) and published application (US 2002/0157139 A1) disclose a method for soybean transformation using a wounded embryonic axis isolated from germinating seed as target for Agrobacterium-mediated transformation. The explant is cultured in a medium containing a selection agent. The difference between the granted patent and the application is that in the patent application the wounding step is omitted, thereby providing an alternative protocol to obtain transgenic soybean plants.
In the European and Australian applications, Monsanto discloses a method to prepare a transformed germline of soybean starting with an embryonic axis of a soybean seed for transformation process.
The inventions disclosed by Pioneer Hi-Bred in patents granted in the United States (2), Australia (1) and Canada (1) use a hypocotyl or a cotyledonary node of a soybean seed transformed with any species of Agrobacterium. Detailed protocols for co-cultivation of a soybean explant with the bacterium are also claimed.
University of Mississippi owns a United States patent (US 5968830) on an Agrobacterium-mediated transformation method of a hypocotyl soybean explant, which also recites a method of regenerating soybean via organogenesis.
The information contained in this page was believed to be correct at the time it was collated. New patents and patent applications, altered status of patents, and case law may have resulted in changes in the landscape. CAMBIA makes no warranty that it is correct or up to date at this time and accepts no liability for any use that might be made of it. Corrections or updates to the information are welcome. Please send an email to info@bios.net.
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