Sorghum - Specific Patent Information

A method for transforming sorghum with a nucleotide sequence of interest, said method comprising the steps of:

A) contacting tissue selected from the group consisting of: an immature embryo or cells derived from an immature embryo, immature inflorescence, the basal portion of young leaves and tissue capable of forming callus and/or secondary embryos from a sorghum plant with an Agrobacterium comprising a vector which comprises said nucleotide sequence, wherein said nucleotide sequence comprises at least an expression cassette comprising a gene which confers resistance to a selection agent;
B) co-cultivating the tissue with said Agrobacterium in a concentration from about 1 x 10³ cfu/ml to about 1.5 x 10¹⁰ cfu/ml;
C) culturing the tissue in a medium comprising an antibiotic capable of inhibiting the growth of Agrobacterium and said selection agent;
D) regenerating transformed sorghum plants.

A method for transforming sorghum with a nucleotide sequence, said method comprising the steps of:

A) contacting a tissue selected from the group consisting of: an immature embryo or cells derived from an immature embryo, immature inflorescence, the basal portion of young leaves and tissue capable of forming callus and/or secondary embryos from a sorghum plant with an Agrobacterium comprising a super-binary vector which comprises said nucleotide sequence, wherein said nucleotide sequence comprises at least an expression cassette co mprising a gene which confers resistance to a selection agent;
B) co-cultivating the tissue with said Agrobacterium in a concentration from about 1 x 10³ cfu/ml to about 1.5 x 10¹⁰ cfu/ml;
C) culturing the tissue in a medium comprising an antibiotic capable of inhibiting the growth of Agrobacterium and said selection agent;
D) regenerating transformed sorghum plants.

A Agrobacterium-mediated transformed sorghum plant, said plant comprising fewer than 5 copies of a nucleic acid of interest flanked by at least one T-DNA border sequence incorporated in its genome, wherein said plant is derived from tissue selected from the group consisting of: an immature embryo or cells derived from an immature embryo, immature inflorescence, the basal portion of young leaves and tissue capable of forming callus and/or secondary embryos.

A Agrobacterium-mediated transformed plant cell, said cell comprising fewer than 5 copies of a nucleic acid of interest flanked by at least one T-DNA border sequence incorporated in its genome, wherein said plant cell is sorghum and wherein said plant cell is derived from tissue selected from the group consisting of: an immature embryo or cells derived from an immature embryo, immature inflorescence, the basal portion of young leaves and tissue capable of forming callus and/or secondary embryos.

A Agrobacterium-mediated transformed plant tissue, said tissue comprising fewer than 5 copies of a nucleic acid of interest flanked by at least one T-DNA border sequence incorporated in its genome of cells of said tissue, wherein said plant is sorghum and wherein said plant tissue is derived from tissue selected from the group consisting of: an immature embryo or cells derived from an immature embryo, immature inflorescence, the basal portion of young leaves and tissue capable of forming callus and/or secondary embryos.

A method for transforming sorghum with a nucleotide sequence of interest, said method comprising the steps of: contacting an immature embryo from a sorghum plant with an Agrobacterium comprising a vector which comprises said nucleotide sequence, wherein said nucleotide sequence comprises at least an expression cassette comprising a gene which confers resistance to a selection agent; co-cultivating said immature embryo with said Agrobacterium; culturing said immature embryo in a medium comprising an antibiotic capable of inhibiting the growth of said Agrobacterium and said selection agent; regenerating transformed sorghum plants.

A method for transforming sorghum with a nucleotide sequence, said method comprising the steps of: contacting an immature embryo from a sorghum plant with an Agrobacterium comprising a super-binary vector which comprises said nucleotide sequence, wherein said nucleotide sequence comprises at least an expression cassette comprising a gene which confers resistance to a selection agent; co-cultivating said immature embryo with said Agrobacterium; culturing said immature embryo in a medium comprising an antibiotic capable of inhibiting the growth of said Agrobacterium and said selection agent; regenerating transformed sorghum plants.

An Agrobacterium-transformed sorghum plant wherein said plant comprises fewer than 5 copies of a nucleic acid of interest flanked by at least one T-DNA border sequence incorporated in its genome.

A sorghum plant transformed by contacting an immature embryo from a sorghum plant with an Agrobacterium comprising a nucleic acid of interest; co-cultivating said immature embryo with said Agrobacterium; culturing said immature embryo in a medium comprising an antibiotic capable of inhibiting the growth of said Agrobacterium; and regenerating a transformed sorghum plant, said plant comprising fewer than 5 copies of said nucleic acid flanked by at least one T-DNA border sequence incorporated in its genome.

A sorghum plant transformed by contacting an immature embryo from a sorghum plant with an Agrobacterium comprising a vector which comprises; a nucleic acid comprising at least one expression cassette comprising a gene of interest; and a nucleic acid comprising at least one expression cassette comprising a gene which confers resistance to a selection agent; co-cultivating said immature embryo with said Agrobacterium; culturing said immature embryo in a medium comprising an antibiotic capable of inhibiting the growth of said Agrobacterium and a selection agent; and regenerating a transformed sorghum plant, said plant comprising fewer than 5 copies of said vector flanked by at least one T-DNA border sequence incorporated in its genome.