Weight reduction of the transformed plant tissue
Patent applications filed by Monsanto
During co-cultivation of plant tissue with Agrobacterium it is
desirable to reduce the weight of the explant in order to facilitate the DNA
transfer process and the formation of embryogenic callus.
Monsanto's disclosure teaches methods for
reducing the weight of the explant during the
co-cultivation period. Preferred methods of the inventors include
reduction of moisture conditions, applying vacuum, increasing the osmotic
potential of the media by use of mannitol, sorbitol or polyethylene glycol, air
drying the explant by evaporation or applied air, or applying chemicals
(desiccants) such as calcium oxide to extract moisture from the explant.
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
2001/054186 AA
- Earliest priority - 11 December 1998
- Filed - 10 December 1999
- Published - 20 December 2001
- Expected expiry - N/A
- Status -pending
WO
200034491 A2
- Earliest priority - 11 December 1998
- Filed - 9 December 1999
- OPI - 15 June 2000
- Expected expiry - N/A
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Title - An improved efficiency
Agrobacterium-mediated plant transformation method
Claim 1
A method for producing a fertile transgenic plant, comprising the steps of:
(a) introducing one or more genetic component(s) one desires to introduce
into the genome of a plant by co-culturing a regenerable plant cell or tissue
with Agrobacterium containing said genetic component(s);
(b)
co-culturing said Agrobacterium and regenerable plant cells or tissues
of step (a) under conditions that decrease the weight of said
Arobacterium-inoculated explant;
(c) identifying or selecting a
transformed cell line; and
(d) regenerating a fertile transgenic plant
therefrom.
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The claims as filed of both the United States and PCT applications recite a
method for producing a fertile transgenic plant by co-culturing a plant cell or
tissue with Agrobacterium having the genes of interest under conditions
that decrease the weight of the explant. The transformed cell lines are selected
and regenerated into a fertile transgenic plant.
According to the specification, the method to reduce the weight of the
inoculated explant 'comprises any method that reduces the weight of the
inoculated explant and facilitates the DNA transfer process.'
Preferred methods to reduce the weight of the explant are
(but not limited to):
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restricting exogenous moisture to the explant during co-culture
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reducing the weight of the explant by applying a vacuum during
co-culture
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increasing the osmotic potential of the media, for example, by use of
mannitol, sorbitol, raffinose, or polyethylene glycol or combinations
thereof
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air drying the explant to reduce the weight of the explant by
evaporation or applied air
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chemical means of extracting moisture from the explant during
co-culture, for example, by placing the explant in a dessicating
environment
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Monsanto
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AU
773764
- Earliest priority - 11 December 1998
- Filed - 9 December 1999
- Granted - 3 June 2004
- Expected expiry - 8 December 2019
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Title - An improved efficiency
Agrobacterium-mediated plant transformation method
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Claim 1
A method for producing a fertile transgenic plant, comprising the steps of:
(a) introducing one or more genetic component(s) into the genome of a plant
by co-culturing a regenerable plant cell or tissue with Agrobacterium
containing said genetic component(s);
(b) co-culturing said
Agrobacterium and regenerable plant cells or tissues of step (a) in a
manner controlling a reduction in the weight of said
Agrobacterium-inoculated explant of from about 20% to 35% of the weight
of the plant cell or tissue prior to co-culture, wherein the manner for
controlling a reduction in the weight of the Agrobacterium-inoculted
explant comprises limitation or removal of water from the explant;
(c) identifying or selecting a transformed cell line; and
(d)
regenerating a fertile transgenic plant therefrom.
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Granted AU 773764 recites a method for producing a fertile
transgenic plant using Agrobacterium, where the method of weight
reduction of the inoculated explant is limited to 'limitation or removal of
water from the explant'.
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| Remarks |
- National phase entries of
WO
2000/34491 in Canada (CA 2353796), Europe (EP 1137790) and Japan (JP
2002531132) are pending.
- Other national phase entries of
WO
2000/34491 include: Brazil (BR 9916103), South Africa (ZA 200104091).
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Note: Patent information on this page was last updated on 7 March 2006.
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