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US
6323396 B1
- Earliest priority - 25 August 1997
- Filed - 24 February 2000
- Granted - 27 November 2001
- Expected expiry - 23 February 2020
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Title - Agrobacterium-mediated transformation of
plants
Claim 1
A process to produce a dicotyledonous transgenic plant, said plant comprising
- a foreign DNA fragment integrated into the genome of at least some of its
cells, said process comprising the following steps:
1) providing a plant which is systemically infected with an
Agrobacterium strain auxotrophic for methionine or cysteine, or adenine
and histidine, harboring a DNA of interest which is operably linked to at least
one T-DNA border sequence; and
2) generating a transgenic plant from a
single cell or a group of cells isolated from said systemically infected plant.
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Claim 11
Bacterial strain LBA4404metHV, deposited as LMG P-18486.
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Claim 12
Bacterial strain ATHV ade,his, deposited as LMG P-18485.
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Claim 13
A method for producing a transgenic plant, said plant comprising
- a foreign DNA fragment integrated into the genome of at least some of its
cells, said process comprising:
a. cocultivating a plant cell, plant tissue, explant or plant with an
Agrobacterium strain auxotrophic for methionine or cysteine, or for
adenine and histidine, to generate a transgenic cell; and
b. regenerating a
transgenic plant from said transgenic cell.
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The United States patent
US
6323396 B1 claims
- A process for the production of transgenic plants (Claims 1 and 13)
utilizing an Agrobacterium strain deficient in the biosynthesis of one
of the following:
- methionine
- cysteine
- adenosine and histidine
- Co-cultivating a plant or plant tissue with one of the auxotrophic
Agrobacterium mutants described above carrying a desired foreign DNA
and regenerating a transgenic plant from such a treated tissue (Claim 13).
- Systemically infecting a plant with one of the auxotrophic
Agrobacterium mutants described above carrying a desired foreign DNA
and regenerating a transgenic plant from such a treated tissue (Claim 1).
- Two auxotrophic Agrobacterium strains as described (Claims 11 and
12).
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Nunhems Zadens B.V.
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AU
736349 B2
- Earliest priority - 25 August 1997
- Filed - 25 August 1998
- Granted - 26 July 2001
- Expected expiry - 24 August 2018
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Title - Improved Agrobacterium-mediated
transformation of plants
Claim 1
A process to produce a transgenic plant comprising
- a foreign DNA fragment integrated into the genome of at least some of its
cells, said process comprising the following steps:
1) providing a plant which is systemically infected with an auxotrophic
Agrobacterium strains harbouring a DNA of interest which is operably
linked to at least one T-DNA border sequence; and
2) generating a
transgenic plant from a single cell or a group of cells isolated from said
systemically infected plant.
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Claim 12
Bacterial strain LBA4404met.
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Claim 13
Bacterial strain ATHVade, his.
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Claim 14
The use of an Agrobacterium strain auxotrophic for methionine or
cysteine or for adenine and histidine in Agrobacterium-mediated
transformation of plants.
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Claim 15
The use of Agrobacterium strain auxotrophic for
methionine or cysteine or for adenine and histidine in Agrobacterium
mediated transformation of callus from sugarbeet.
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Claim 16
The use of Agrobacterium strain auxotrophic for
methionine or cysteine or for adenine and histidine in Agrobacterium
mediated transformation of corn protoplasts.
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Claim 1 in granted AU 736349 does not have a limit to the
type of nutrient (or nutrients) the Agrobacterium strain is auxotrophic
for.
"Systemically infected plant" is defined in the specification as 'a plant
wherein the Agrobacterium strains is present in at least some part of the
plant'.
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EP
1009844 B1
- Earliest priority - 25 August 1997
- Filed - 25 August 1998
- Granted - 16 June 2004
- Expected expiry - 24 August 2018
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Title - Improved Agrobacterium-mediated
transformation of plants
Claim 1
A process to produce a transgenic plant comprising
- a foreign DNA fragment integrated into the genome of at least some of its
cells, said process comprising the following steps:
1) providing a plant which is systemically infected with an auxotrophic
Agrobacterium strain harbouring a DNA of interest which is operably
linked to at least one T-DNA border sequence; and
2) generating a
transgenic plant from a single cell or a group of cells isolated from said
systemically infected plant.
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Claim 12
Bacterial strain LBA4404metHV, deposited as BCCM/LMG P-18486.
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Claim 13
Bacterial strain ATHV ade,his, deposited as BCCM/LMG
P-18485.
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Claim 14
The use of an Agrobacterium strain auxotrophic for methionine or
cysteine, or for adenine and histidine, in Agrobacterium-mediated
transformation of plants.
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Claim 15
The use of an Agrobacterium strain auxotrophic for
methionine or cysteine, or for adenine and histidine, in Agrobacterium
mediated transformation of callus from sugarbeet.
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Claim 16
The use of an Agrobacterium strain auxotrophic for
methionine or cysteine, or for adenine and histidine, in Agrobacterium
mediated transformation of corn protoplasts.
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Designated contracting States at the time of grant are: Austria (patent
lapsed as reported by INPADOC), Belgium (patent lapsed as reported by EPO),
Switzerland (patent lapsed as reported by INPADOC), Cyprus, Germany (patent
lapsed as reported by EPO), Denmark (patent lapsed as reported by EPO), Spain
(patent lapsed as reported by EPO), Finland (patent lapsed as reported by EPO),
France (patent lapsed as reported by EPO), United Kingdom (patent lapsed as
reported by INPADOC), Greece (patent lapsed as reported by EPO), Ireland (patent
lapsed as reported by INPADOC), Italy, Liechtenstein (patent lapsed as reported
by INPADOC), Luxembourg, Monaco (patent lapsed as reported by EPO), Netherlands
(patent lapsed as reported by INPADOC), Portugal, Sweden (patent lapsed as
reported by INPADOC)
Granted EP 1009844 recites the same method of
Agrobacterium-mediated transformation as that of AU
736349.
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WO
1999/010512 A1
- Earliest priority - 25 August 1997
- Filed - 25 August 1998
- OPI - 4 March 1999
- Predicted expiry - N/A
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Title - Improved Agrobacterium-mediated
transformation of plants
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Claim 1
A process to produce a transgenic piant comprising a foreign DNA fragment
integrated into the genome of at least some of its cells, said process
comprising the following steps:
1) providing a plant which is systemically
infected with an auxotrophic Agrobacterium strain harbouring a DNA of
interest which is operably linked to at least one T-DNA border sequence;
and
2) generating a transgenic plant from a single cell or a group of cells
isolated from said systemically infected plant.
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Claim 12
Bacterial strain LBA44O4met.
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Claim 13
Bacterial strain ATHVade, his.
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Claim 14
The use of an Agrobacterium strain auxotrophic for methionine or
cysteine or for adenine and histidine in Agrobacterium-mediated
transformation of plants.
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Claim 15
The use of Agrobacterium strain auxotrophic for
methionine or cysteine or for adenine and histidine in Agrobacterium
mediated transformation of calius from sugarbeet.
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Claim 16
The use of Agrobacterium strain auxotrophic for
methionine or cysteine or for adenine and histidine in Agrobacterium
mediated transformation of corn protoplasts.
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