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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6162965
- Earliest priority - 2 June 1997
- Filed - 2 June 1998
- Granted - 19 December 2000
- Expected expiry - 1 June 2018
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Title - Plant transformation methods
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Claim 1
A method for transforming a plant cell or
tissue with a gene construct, comprising heat shocking said plant cell or tissue
before co-cultivating with Agrobacterium, wherein said heat shock
treatment inhibits Agrobacterium induced necrosis in said plant cell or
tissue, and said Agrobacterium comprises a vector comprising said gene
construct.
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Claim 2
A method for producing a fertile transgenic plant comprising a gene
construct, which method comprises: (a) transforming a plant cell or tissue
comprising heat shocking said plant cell or tissue before co-cultivating with
Agrobacterium, wherein said heat shock treatment inhibits
Agrobacterium induced necrosis in said plant cell or tissue and said
Agrobacterium comprises a vector comprising said gene construct; and
(b) regenerating the transformed plant cell or tissue to produce said fertile
transgenic plant.
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Claim 12
A method for transforming a maize cell or
tissue with a gene construct, comprising heat shocking said maize cell or tissue
before co-cultivating with Agrobacterium, wherein said heat shock
treatment inhibits Agrobacterium induced necrosis in said maize cell or
tissue, and said Agrobacterium comprises a vector comprising said gene
construct.
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Claim 13
A method for producing a fertile transgenic
maize plant comprising a gene construct, which method comprises: (a)
transforming a maize cell or tissue comprising heat shocking said maize cell or
tissue before co-cultivating with Agrobacterium, wherein said heat
shock treatment inhibits Agrobacterium induced necrosis in said maize
cell or tissue, and said Agrobacterium comprises a vector comprising
said gene construct; and (b) regenerating the transformed maize cell or tissue
to produce said fertile transgenic maize plant.
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Claim 14
A method for transforming a plant cell or tissue with a gene construct,
comprising exposing said plant cell or tissue to Agrobacterium under
conditions which inhibit Agrobacterium induced necrosis, wherein said
conditions comprise delivering to or expressing in said plant cell or tissue a
nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene,
said delivery or expression of said nucleotide sequence inhibits
Agrobacterium induced necrosis in said plant cell or tissue, and said
Agrobacterium comprises a vector comprising said gene construct.
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Claim 15
A method for producing a fertile transgenic plant comprising a gene
construct, which method comprises: (a) transforming a plant cell or tissue
comprising exposing said plant cell or tissue to Agrobacterium under
conditions which inhibit Agrobacterium induced necrosis, wherein said
conditions comprise delivering to or expressing in said plant cell or tissue a
nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene,
said delivery or expression of said nucleotide sequence inhibits
Agrobacterium induced necrosis in said plant cell or tissue, and said
Agrobacterium comprises a vector comprising said gene construct; and
(b) regenerating the transformed plant cell or tissue to produce said fertile
transgenic plant.
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Claim 24
A method for transforming a maize cell or
tissue with a gene construct, comprising exposing said maize cell or tissue to
Agrobacterium under conditions which inhibit Agrobacterium
induced necrosis, wherein said conditions comprise delivering to or expressing
in said maize cell or tissue a nucleotide sequence comprising a coding sequence
of a p35, iap or dad-1 gene, said delivery or expression of said nucleotide
sequence inhibits Agrobacterium induced necrosis in said maize cell or
tissue, and said Agrobacterium comprises a vector comprising said gene
construct.
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Claim 25
A method for producing a fertile transgenic
maize plant comprising a gene construct, which method comprises: (a)
transforming a maize cell or tissue comprising exposing said maize cell or
tissue to Agrobacterium under conditions which inhibit
Agrobacterium induced necrosis, wherein said conditions comprise
delivering to or expressing in said maize cell or tissue a nucleotide sequence
comprising a coding sequence of a p35, iap or dad-1 gene, said delivery or
expression of said nucleotide sequence inhibits Agrobacterium induced
necrosis in said maize cell or tissue, and said Agrobacterium
comprises a vector comprising said gene construct; and (b) regenerating the
transformed maize cell or tissue to produce said fertile transgenic maize plant.
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Claim 26
A method for transforming a gramineceous plant
cell or tissue with a gene construct, comprising exposing said plant cell or
tissue to Agrobacterium under conditions which inhibit
Agrobacterium induced necrosis, wherein said conditions comprise
culturing said plant cell or tissue in a necrosis inhibiting medium, said
necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver
nitrate, or (ii) an ethylene synthesis inhibitor, and said
Agrobacterium comprises a vector comprising said gene construct.
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Claim 27
A method for producing a fertile transgenic
gramineceous plant comprising, a gene construct, which method comprises: (a)
transforming a gramineceous plant cell or tissue comprising exposing said plant
cell or tissue to Agrobacterium under conditions which inhibit
Agrobacterium induced necrosis, wherein said conditions comprise
culturing said plant cell or tissue in a necrosis inhibiting medium, said
necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver
nitrate, or (ii) an ethylene synthesis inhibitor, and said
Agrobacterium comprises a vector comprising said gene construct; and
(b) regenerating the transformed plant cell or tissue to produce said fertile
transgenic gramineceous plant.
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Claim 33
A method for transforming a maize cell or
tissue with a gene construct, comprising exposing said maize cell or tissue to
Agrobacterium under conditions which inhibit Agrobacterium
induced necrosis, wherein said conditions comprise culturing said maize cell or
tissue in a necrosis inhibiting medium, said necrosis inhibiting medium
comprises (i) an ethylene inhibitor other than silver nitrate, or (ii) an
ethylene synthesis inhibitor, and said Agrobacterium comprises a vector
comprising said gene construct.
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Claim 34
A method for producing a fertile transgenic
maize plant comprising a gene construct, which method comprises: (a)
transforming a maize cell or tissue comprising exposing said maize cell or
tissue to Agrobacterium under conditions which inhibit
Agrobacterium induced necrosis, wherein said conditions comprise
culturing said maize cell or tissue in a necrosis inhibiting medium, said
necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver
nitrate, or (ii) an ethylene synthesis inhibitor, and said
Agrobacterium comprises a vector comprising said gene construct.; and
(b) regenerating the transformed maize cell or tissue to produce said fertile
transgenic maize plant.
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Claim 35
A transgenic plant, plant tissue or plant cell comprising a nucleotide
sequence of heterologous origin which comprises a coding sequence of a p35, iap
or dad-1 gag.
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Claim 36
A transgenic plant, plant tissue or plant cell comprising a genome having a
stably integrated nucleotide sequence of heterologous origin which comprises a
coding sequence of a p35, iap or dad-1 gene.
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Claim 40
A transgenic maize plant, tissue or cell
comprising a genome having a stably integrated nucleotide sequence of
heterologous origin comprising a coding sequence of a p35 gene.
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Claim 41
A transgenic maize plant, tissue or cell
comprising a genome having a stably integrated nucleotide sequence of
heterologous origin which comprises a coding sequence of an iap gene.
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Claim 42
A transgenic maize plant, tissue or cell
comprising a genome having a stably integrated nucleotide sequence of
heterologous origin which comprises a coding sequence of a dad-1 gene.
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Claim 44
A gramineceous plant cell or tissue culture
medium, comprising (a) (i) an ethylene inhibitor other than silver nitrate, or
(ii) an ethylene synthesis inhibitor; and (b) an Agrobacterium
comprising a plasmid comprising a gene construct.
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United States patent US 6162965 claims several methods to
inhibit AIN in plants:
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heat shock to treat plant cells or tissues before
co-cultivating with Agrobacterium
- transformation of a plant cell via Agrobacterium with
sequences such as:
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p35 and iap, which are
apoptosis-inhibiting genes from baculovirus; and
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dad-1, a gene capable of suppressing disease response in
plants.
The patent also claims methods for inhibiting AIN in
Gramineae in general and in maize in
particular. Besides the methods mentioned above, gramineaceous plants and maize
may be cultured in a necrosis inhibiting medium containing an inhibitor of
ethylene or ethylene biosynthesis.
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Syngenta
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US
2002/88029 AA
- Earliest priority - 2 June 1997
- Filed - 19 December 2000
- Publication date - 4 Jul 2002
- Expected Expiry - N/A
- Status -Application abandoned 25 July 2005
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Title - Plant transformation methods
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Claim 1
A method of transforming a plant cell with a gene of interest, comprising
- exposing said plant cell to Agrobacterium under conditions which
inhibit Agrobacterium induced necrosis (AIN), wherein said
Agrobacterium comprises a vector comprising said gene of interest.
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Claim 8
A method of making a fertile, transgenic plant comprising
- transforming plant tissue by exposing the tissue to Agrobacterium
under conditions which inhibit Agrobacterium induced necrosis (AIN) and
regenerating tissue thus transformed, wherein said Agrobacterium
comprises a vector comprising a gene of interest.
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Claim 9
A plant, plant tissue or plant cell comprising a nucleotide sequence of
heterologous origin which inhibits AIN.
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Claim 10
A plant cell or tissue culture medium, comprising
a) a chemical inhibitor of AIN,
b) an Agrobacterium comprising
a plasmid comprising a gene of interest, and
c) water and essential salts.
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Claim 11
A method of transforming a totipotent cell of a plant of the
family Gramineae, comprising exposing a population of said totipotent cells to
Agrobacterium comprising a plasmid comprising a gene of interest,
wherein the Agrobacterium is of a strain which does not induce
significant levels of necrosis in said population at an exposure duration and
concentration sufficient to achieve transformation of said cell.
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Claim 12
A method for determining the suitability of an
Agrobacterium strain for use in the transformation of a regenerable
cell of a plant of the family Gramineae comprising exposing a population of said
regenerable cells of the plant to the Agrobacterium strain and
observing the necrosis in said cell population.
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Claim 13
An Agrobacterium strain which has been genetically modified to
reduce or eliminate expression of the Agrobacterium necrosis factor or
a derivative of such a modified strain.
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United States application US 2002/88029 is a continuation of
application No. 09/089,111, which is now patent US 6162965
described above. The additional elements in this application include a method to
select an Agrobacterium strain with reduced necrosis-inducing capacity
from a population of regenerable plant cells from the family Gramineae. Further,
the application also claims the Agrobacterium strain with reduced or
eliminated production of the necrosis factor, obtained through the selection
process or through genetic manipulation. Finally, a method is claimed to
transform regenerable cells from the family Gramineae using such strains.
The "Agrobacterium necrosis factor" is disclosed as "the heat labile factor
observed in concentrated supernatant capable of inducing necrosis, e.g.,
programmed cell death, in maize embryos." The application discloses sequences
cloned into three BAC vectors associated with affects on cell death. The
sequences are reported to have homology with virB1,
xylA-xylB and acvB respectively. It's not clear if the
Agrobacterium strain referred to in claim 13 is required to reduce or
eliminate the expression of one or all of these sequences since none of these
sequences are clearly identified as "the" factor. The genetic structure of a
strain having the properties recited in the claims is not disclosed nor is a way
of identifying such a strain disclosed.
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AU
735472 B
- Earliest priority - 2 June 1997
- Filed - 29 May 1998
- Granted - 12 July 2001
- Expected expiry - 28 May 2018
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Title - Plant transformation methods
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Claim 1
A method of transforming a plant cell or tissue with a gene of interest,
comprising:
- exposing said plant cell or tissue to Agrobacterium under
conditions which inhibit Agrobacterium-induced necrosis (AIN), wherein
said Agrobacterium comprises a vector comprising said gene of
interest, wherein
said conditions which inhibit AIN comprise:
- exposing said plant cell or tissue to Agrobacterium after heat
shock treatment; or
- exposing said plant cell or tissue to Agrobacterium in the presence
of an agent inhibiting AIN, wherein said agent comprises:
- a chemical inhibitor, wherein said chemical inhibitor is a compound selected
from the group consisting of ethylene inhibitors other than silver nitrate,
ethylene synthesis inhibitors, gibberellin antagonists, and phosphatase
inhibitors; or
- a nucleotide sequence encoding mRNA or protein inhibiting AIN.
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| Claim 18
A method of making a fertile, transgenic plant comprising: A) transforming
plant tissue by exposing the tissue to Agrobacterium under conditions
which inhibit Agrobacterium -induced necrosis (AIN); and
B)
regenerating tissue thus transformed, wherein
said Agrobacterium
comprises a vector comprising said gene of interest, wherein
said
conditions which inhibit AIN comprise:
- exposing said plant cell or tissue to Agrobacterium after heat
shock treatment; or
- exposing said plant cell or tissue to Agrobacterium in the presence
of an agent inhibiting AIN, wherein said agent comprises:
- a chemical inhibitor, wherein said chemical inhibitor is a compound selected
from the group consisting of ethylene inhibitors other than silver nitrate,
ethylene synthesis inhibitors, gibberellin antagonists, and phosphatase
inhibitors; or
- a nucleotide sequence encoding mRNA or protein inhibiting AIN.
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| Claim 39
A plant, plant tissue or plant cell comprising a nucleotide sequence of
heterologous origin which inhibits AIN.
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| Claim 50
A plant cell or tissue culture medium, comprising:
- a chemical inhibitor, wherein said chemical inhibitor is a compound selected
from the group consisting of ethylene inhibitors other than silver nitrate,
ethylene synthesis inhibitors, gibberellin antagonists, and phosphatase
inhibitors;
- an Agrobacterium comprising a plasmid comprising a gene of
interest; and
- water and essentials salts.
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The methods for inhibiting AIN claimed in the granted Australian
patent are similar to the ones claimed in the
United States patent. However, the group of chemical inhibitors
additionally includes gibberellin antagonists and
phosphatase inhibitors. Also, the nucleotide sequences whose
products inhibit AIN are not limited to specific genes, but encode any
mRNA or protein inhibiting AIN. Furthermore, unlike
the United States patent, the Australian patent does
not claim transformation of Gramineae or maize in particular.
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WO
1998/54961 A2
- Earliest priority - 2 June 1997
- Filed - 29 May 1998
- Publication date - 10 December 1998
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Title - Plant transformation methods
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Claim 1
A method of transforming a plant cell with a gene of interest, comprising
- exposing said plant cell to Agrobacterium under conditions which
inhibit Agrobacterium induced necrosis (AIN), wherein said
Agrobacterium comprises a vector comprising said gene of interest.
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Claim 8
A method of making a fertile, transgenic plant comprising
- transforming plant tissue by exposing the tissue to Agrobacterium
under conditions which inhibit Agrobacterium induced necrosis (AIN) and
regenerating tissue thus transformed, wherein said Agrobacterium
comprises a vector comprising a gene of interest.
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Claim 9
A plant, plant tissue or plant cell comprising a nucleotide sequence of
heterologous origin which inhibits AIN.
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Claim 10
A plant cell or tissue culture medium, comprising
a) a chemical inhibitor of AIN,
b) an Agrobacterium comprising a plasmid
comprising a gene of interest, and
c) water and essential salts.
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Claim 11
A method of transforming a totipotent cell of a plant of the
family Gramineae, comprising exposing a population of said totipotent cells to
Agrobacterium comprising a plasmid comprising a gene of interest,
wherein the Agrobacterium is of a strain which does not induce
significant levels of necrosis in said population at an exposure duration and
concentration sufficient to achieve transformation of said cell.
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Claim 12
A method for determining the suitability of an
Agrobacterium strain for use in the transformation of a regenerable
cell of a plant of the family Gramineae comprising exposing a population of said
regenerable cells of the plant to the Agrobacterium strain and
observing the necrosis in said cell population.
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Claim 13
An Agrobacterium strain which has been genetically modified to
reduce or eliminate expression of the Agrobacterium necrosis factor or
a derivative of such a modified strain.
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The claims as filed in the PCT application are
broader in scope than the granted claims of both the Australian
and the US patents. For example, one claim broadly recites "conditions which
inhibit AIN" without specifying details of suitable conditions. In another claim
the culture medium for the plant cell or tissue contains a chemical inhibitor
without defining what sort of chemical. A couple of claims of the European
application make reference to the use of an Agrobacterium
strain that does not induce significant levels of
necrosis. The Agrobacterium strain has been modified
to reduce or eliminate expression of a necrosis factor. This strain of
Agrobacterium may be used for transforming a totipotent Gramineae cell.
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| Remarks |
- National phase entries of WO 1998/54961 in Canada (CA 2290863), Europe (EP
986299) and Japan (JP 2002502252) are pending.
- National phase entry of WO 1998/54961 in China (CN 1150320) has been granted
on 19 May 2004.
- Other national phase entries listed in INPADOC include: Hungary (HU
200002903), Israel (IL 132768), Poland (PL 336979), Russian Federation (RU
2226549), Turkey (TR 200402566), Ukraine (UA 72443), South Africa (ZA 9804681).
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