Callus Transformation - patents granted to Japan Tobacco
Specific patent information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5591616 A
- Earliest priority - 7 July 1992
- Filed - 3 May 1994
- Granted - 7 January 1997
- Expected expiry - 6 July 2013
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Title - Method of transforming monocotyledons
Claim 1
A method for transforming a monocotyledon callus, comprising contacting a
cultured tissue of a monocotyledon during dedifferentiation wherein said
dedifferentiation is obtained by culturing an explant on a
dedifferentiation-inducing medium for not less than 7 days or a dedifferentiated
cultured tissue of a monocotyledon, with a bacterium belonging to the genus
Agrobacterium containing a desired gene.
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Claim 17
A method for transforming a monocotyledon with a desired gene, comprising:
contacting a cultured tissue of said monocotyledon during dedifferentiation
thereof, or a dedifferentiated cultured tissue of said monocotyledon, with a
suspension of Agrobacterium tumefaciens having a cell population of
106 to 1011 cells/ml for 3-10 minutes and then culturing
said cultured tissue of said monocotyledon during dedifferentiation thereof, or
said dedifferentiated cultured tissue of said monocotyledon, on a solid medium
for several days together with said Agrobacterium tumefaciens, or
adding said Agrobacterium tumefaciens to culture medium in which said
cultured tissue of said monocotyledon during dedifferentiation thereof or said
dedifferentiated cultured tissue of said monocotyledon is cultured, and
continuously culturing said cultured tissue of said monocotyledon during
dedifferentiation or said dedifferentiated cultured tissue of said monocotyledon
together with said Agrobacterium tumefaciens, wherein said
dedifferentiated cultured tissue of said monocotyledon is selected from the
group consisting of a tissue cultured during the process of callus formation
which is cultured for not less than 7 days after an explant is placed on a
dedifferentiation-inducing medium and a callus, and wherein said
Agrobacterium tumefaciens contains plasmid pTOK162, and said desired
gene is present between border sequences of the T region of said plasmid
pTOK162, or wherein said desired gene is present in another plasmid contained in
said Agrobacterium tumefaciens.
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US 5591616 claims:
- a method for transforming monocotyledon callus by contacting
dedifferentiating tissue of not less than 7 days of culture or
dedifferentiated tissue with Agrobacterium having a desired gene; and
- a method of transforming such tissue or transforming a callus by
contacting the tissue/callus with a suspension of Agrobacterium cells
of 106 - 1011 cells/ml for 3-10 minutes. The bacterium
contains the desired gene either between the T- borders of the plasmid pTOK162
or in another plasmid.
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Japan Tobacco
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EP 604662 A1
- Earliest priority - 7 July 1992
- Filed - 6 July 1994
- Application pending
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Title - Method of transforming monocotyledon
Claim 1
A method for transforming a monocotyledon comprising transforming a cultured
tissue during dedifferentiation process or a dedifferentiated cultured tissue of
said monocotyledon with a bacterium belonging to genus Agrobacterium
containing a desired gene.
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The claims submitted in the European application EP 604662
A1 are the same as the claims of the Australian patent, below.
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AU 667939 B
- Earliest priority - 7 July 1992
- Filed - 6 July 1993
- Granted - 18 April 1996
- Expected expiry- 6 July 2013
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Title - Method of transforming monocotyledon
Claim 1
A method for transforming a monocotyledon comprising transforming a cultured
tissue during dedifferentiation process or a dedifferentiated cultured tissue of
said monocotyledon with a bacterium belonging to genus Agrobacterium
containing a desired gene.
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The lead claim in the Australian patent AU 667 939 is broader than in the
United States patent. In the Australian patent, a dedifferentiating or
dedifferentiated tissue of a monocot is also used as the initial tissue for
transformation, but there is no restriction on the number of days of
culture in the medium to induce dedifferentiation.
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US
2002/178463 A1
- Earliest priority - 7 July 1992
- Filed - 13 January 1999
- Application pending
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Title - Method for transforming monocotyledons
Claim 1
A method for transforming a monocotyledon, comprising contacting a cultured
tissue of said monocotyledon during dedifferentiation thereof obtained by
culturing an explant on a dedifferentiation-inducing medium for less than 7 days
with a bacterium belonging to the genus Agrobacterium containing a
super binary vector having the virulence region of Ti plasmid pTiBo542 contained
in Agrobacterium tumefaciens A281, left and right border sequences of
T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus
Agrobacterium, and a desired gene located between said left and right
border sequences.
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Claim 13
A method for transforming a monocotyledon, comprising contacting a cultured
tissue of said monocotyledon during dedifferentiation thereof obtained by
culturing an explant derived from an immature tissue on a
dedifferentiation-inducing medium for less than 7 days with a bacterium
belonging to the genus Agrobacterium containing a desired gene and
containing a vector having the virulence region of Ti plasmid contained in
Agrobacterium tumefaciens.
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This application is a continuation of abandoned US 08/668464, which was a
continuation of now granted US 5591616.
Claims in this applicaiton recite Agrobacterium-mediated
transformation of monocotyledon explants, where the explant is cultured on
dedifferentiation medium for less than 7 days, then infected with
Agrobacterium containing a vector that has the virulence region (in
particular a vector that contains the virulence region of Ti plasmid pTiBo542
from A. tumefaciens A281 in the case of claim 1).
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| Remarks |
- National phase entry of WO 1994/00977 in Canada (CA 2121545) is pending.
- National phase entry of WO 1994/00977 in Japan (JP 2649287 B2) has been
published as granted on 3 September 1997.
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Note: Patent information on this page was last updated on 21 February 2006.
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