Algae transformation - Specific Patent
Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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WO
2000/62601 A1
- Earliest priority - 15 April 1999
- Filed - 14 April 2000
- OPI - 26 October 2000
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Title - Agrobacterium-mediated genetic
transformation of multicellular marine algae, resultant strains and their
products
| Claim 1
A method for causing genetic transformation of multicellular marine algae,
said method comprising:
A) culturing cells of a transformation-competent Agrobacterium
species, said cells containing a Ti plasmid that contains a gene of interest;
B) wounding a multicellular marine algae to be transformed in a manner
that is sufficient to penetrate at least the cuticle, or outer cell wall layer
of said alga;
C) applying cells of said transformation-competent
Agrobacterium species to wounded cells of said alga; and
D)
co-culturing said applied cells of said Agrobacterium species with said
wounded algal cells for a time sufficient to effect transformation of some of
said algal cells.
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| Claim 20
A stable transgenic multicellular marine algae, said alga comprising a DNA
sequence coding for a gene foreign to said alga, wherein said alga is further
capable of expressing said DNA sequence and of transferring said expressible DNA
sequence to progeny of said alga.
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| Claim 22
A transgenic strain of marine algae comprising and capable of expressing a
DNA sequence coding for an antigen of a pathogenic microorganism or an antigenic
determinant thereof, wherein said antigen or antigenic determinant thereof is
capable of eliciting a secretory immune response in a human or other animal upon
oral administration of cellular material from said algae.
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| Claim 23
A transgenic strain of marine algae with enhanced disease resistance to
marine fungi compared to a non-transformed said strain.
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Claim 24
A transgenic strain of marine algae capable of producing a compound having an
enhanced health benefit compared to a non-transformed said strain.
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| Claim 26
A method for eliciting a secretory immune response in a human or other
animal, said method comprising:
- orally administering an effective amount of a composition comprising
transgenic marine algae, or tissue thereof,
wherein said transgenic algae,
or algal tissue, comprise and are capable of expressing a DNA sequence coding
for an antigen of a pathogenic microorganism or an antigenic determinant
thereof,
wherein said antigen or antigenic determinant thereof is capable
of eliciting a secretory immune response in a human or other animal upon oral
administration of cellular material from said algae.
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The claims as filed of the present PCT application recite:
- a method for the transformation of multicellular marine algae with
Agrobacterium by
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- wounding the cuticle of the algae
- applying competent Agrobacterium containing a gene of interest to
the wounded alga; and
- co-culturing both Agrobacterium and algal cells allowing enough
time for the transformation process
- a stable transgenic marine alga capable of expressing a foreign sequence and
transferring such sequence to its progeny;
- transgenic strains of marine algae comprising:
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- antigens as elicitors for human or animal immune response
- resistance to marine fungi
- a compound for health benefit
- a method to elicit human or animal immune response by orally administering a
transgenic alga or algal tissue containing an antigen that acts as an elicitor
of an immune response.
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Northeastern University
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| Remark |
The present PCT application has passed the deadline to enter national phase
(30 or 31 months from filing depending on the country), therefore will not have
any patents granted from this particular application.
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Note: Patent information on this page was last updated on 9 February 2006.
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